Comparison between Two Fermentation Media in Production of Antibacterial Crude Extracts from Penicillium Chrysogenum
Keywords:
Penicillium chrysogenum, antimicrobial compounds, PPM, CSLMAbstract
Background: Filamentous fungi such as Penicillium chrysogenum is widely used as hosts for the industrial products such as proteins and secondary metabolites. Aim of study: Determine which two fermentation media used in this study is the best for the production of crude antimicrobials from Penicillium chrysogenum. A strain of Penicillum chrysogenum UNIJAG.PL.242was isolated from water sample (0.5 ml) of the Hammar marsh area in Thi-Qar Province, south of Iraq. The isolation was carried out using potato dextrose agar (PDA) at 25°C for 5 days and sub-culture was conducted on Malt extract agar (MEA) and Czapez dox agar (CDA) besides PDA at the same conditions of the isolation. P. chrysogenum was identified depending on the morphological and molecular characteristics in which ß-tubulin genes within the fungal genome were detected and then sequenced. This fungus was preliminarily tested against Staphylococcus aureas, Streptococcus pyogens, Bacillus cerues, Escherichia coli and Psuedomonus aeroginosa by which P. chrysogenum inhibited growth of Staphylococcus aureas. The fungus fermented using penicillin production medium (PPM) and corn steep liquor medium (CSLM) and the crude extracts of the fungal filtrate were harvested and tested against these bacteria that the extract of PPM inhibited S.aureus, S.pyogenes, B.cerues. And E.coli but P.aeroginosa was resistant to this extract. Relatedly, the extract of CSLM did not produce inhibitory values against B.cerues and P.aeroginosa which the remaining tested bacteria were affected. Both crude extracts were analyzed using HPLC which showed the numbers of peaks which some of them were similar to peak of pure penicillin G which was tested a control in this process. Conclusion: The present study concluded this fungus produced antimicrobial compounds and PPM is better fermentation medium than CSLM.References
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