Cytotoxicity and DNA binding of Manganese (II) complex Containing pyridine ligand
DOI:
https://doi.org/10.32792/jmed.2025.29.5Keywords:
Mn (II), 2-acetyl-6-bromopyridine, oxime, DNA interaction, cytotoxicity, anticancerAbstract
The interaction between large biomolecules and ligands has attractedconsiderable interest due to its biological properties. Calf thymus DNA
(CT-DNA) can interact with biologically active compounds to form
complexes. DNA is a primary target for anticancer drugs that are
involved in important cellular processes.The ligand and the manganese
complex MnCl2 (2-acetyl-6-bromopyridine oxime)2 were synthesized.
The ligand was examined using infrared spectroscopy, CHN, 1HNMR,
and 13CNM techniques. CHN elemental analysis and infrared
spectroscopy were also used to analyze the complex.Both compound 1
and the ligand demonstrated cross-linking with calf thymus DNA (CT
DNA), and the complex demonstrated higher binding efficiency than the
ligand alone. The in vitro toxicity of compound 1 was evaluated using
metabolic assays using 3-(4,5-dimethylthiazol-2-yl)-2,5
diphenyltetrazolium bromide as an anticancer agent against esophageal
cancer cells (SKGT-4). In SKGT-4 cells, the compound showed a
significant inhibitory effect. Therefore, it could be proposed as a future
anticancer drug due to its low cytotoxicity.
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