Effect of propolis extract in inhibiting the growth of bacteria isolated from UTI in patients Of AL-Hussein Teaching Hospital, In Thi-Qar, city

Abstract

Among the most common diseases that afflict the human urinary tract is a bacterial infections (UTIs) is one more common of these injuries involve the lower urinary tract, the bladder and urethra , UTIs are much more common in elderly than other individuals for a different reasons, and frequently occur both in community and hospital environments .The study was aimed at determining the causal microbial agents of urinary tract infection UTI  infections  and the susceptibility of isolates to propolis as a natural antimicrobial substance compared with the antibiotcics used . A total of 25 urine sample was obtained from patients (10 male and 15 female ) who were diagnosed with UTI attending AL- Hussein Teaching Hospital ,Thi-Qar city, Samples were collected between  June and July 2015, cultured and the isolates were characterized by standard microbiological procedures. Of the 25 samples,10 male had positive cultures with E.coli having the highest prevalence followed by 3 Klebsiella species  and 2 isolates were identified as Staphylococci species and 15 female positive results with 7 E.coli  followed by Klebsiella and Staphylococci species same, and evaluating of the inhibitory effect of crud propolis against bacterial isolates. Four graduated concentrations were prepared propolis 12.5, 25, 50, and 100 mg/ml and its activity was checked up by agar well diffusion method. The concentration of propolis exhibit proportionality with zone of inhibition of bacterial isolates. The propolis at concentration 50and 100 mg/ml has significant activity in comparison with antibacterial used in this study at (P<0.001) for each ,Ceftriaxone 30 µg, Amikacin 30 µg and Gentamycin 10 µg respectively which were exhibited best activity from each other antibacterial which have been used.