Study the effect of hyperthermia ,laser and chemotherapy on white blood cell activity in cancers patient measuring by means ofLuminol dependentChemiluminescence
Keywords:
laser, hyperthermia, chemotherapy, Chemiluminescence responseAbstract
Investigation the effects of low-level laser (532nm)irradiation and hyperthermia combination with chemotherapy on white blood cell activity in patient with different type of cancer, measuring by mean of luminol-dependent chemiluminescence(CL).Diminished function activity of peripheral blood neutrophils has been found in untreated human patients with several different types of cancer.As expected, cell viability decreases gradually with time . About 60 min, cell viability was about 75% in the control group, while in the group heated to 40oC, only 30% of the cells are viable. Approximately 60% of cells died after a 30 min exposure at 40oC. Exposure for 60-120 min at 40oC causes a similar effect .Laser radiation causes significant enhancement of spontaneous chemiluminescence response(P<0.05).Un expected result,the increase in CL functional activity has been found with laser treatment for all times of irradiation. A maximum enhancement in CL functional activity has been found at about 60 min of irradiation (P<0.05). A combination of laser and HT has similar behaviorof CL response(P<0.05).The conclusion drown from this in vitro study demonstrated that diode laser radiation (at low power levels laser (λ = 532 nm, I = 150 mW/cm2 ) combination with hyperthermia causes enhancement effects in phagocytosis activity of white blood cell in cancer patient.References
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