Study the association between the infection with Trichomonas vaginalis and use of contraceptive among women with abnormal vaginal discharge by PCR technique in Nassiriyah city
Abstract
The aim of the present study was designed to determine the relationship between the infection with T. vaginalis and use of contraceptive among women whom suffering from abnormal vaginal discharge in Nassirriyah city \ Thiqar Province using TVK3/7 gene as target by PCR technique. all women entered the Maternity and pediatrics hospital , private clinics and laboratories. The results are explain that the infection with T. vaginalis are decrease during the use of contraceptive since there are no case of infection with T. vaginalis among women whom used injection , pills and tubal ligation except there is three or 3(0.67) cases of infection is recorded among women who use of IUCD compared with 11 (3.1) total infection among women whom are not use the contraceptive.References
- Petrin , D. ; Delgaty , K. ; Bhatt , R. and Garber , G. (1998). Clinical and microbiological aspect of Trichomonas vaginalis . Clin. Microbiol. Rev. , 11: 300 – 317.
- Wolner – Hanssen , P. ; Krieger, J.N. ; Stevens, C.E. ; Kiviat, N.B. ; Koutsky, L. ; Derouen, T. ; Hiller, S. and Holmes, K.K. (1989). Clinical manifestation of vaginal Trichomoniasis . JAMA., 261 : 571 – 576.
- Stone, K.M. ; Grimes, D.A. and Magder, L.S. (1986). Primary prevention of sexually transmitted disease . J. Am. Med. Assoc . , 255 : 1763 –1766.
- Schneider , H. ; Coetzee , N. and Fehler , H.G. (1998). Screening for sexually transmitted diseases in ruler South African women . Sex. Trans. Infect. , 74 : 147 – 152.
- Sehgal , VN. (1987). Venereal disease . , 2nd ed., Japee Brothers , New Delhi , 124 – 125.
- Schwebke , J.R. and Burgess , D. (2004). Trichomoniasis . Clin. Microbiol. Rev. ,
: 794 – 803.
- Sena , A.C. ; Miller , W.C. ; Hobbs , M.M. ; Schwebke , J.R. ; Leone , P.A. ; Swygard , H. ; Atashili , J. and Cohen , M.S. (2007). Trichomonas vaginalis infection in male sexual partners : Implication for diagnosis , treatment and prevention . Clin. Infect. Dis. , 44 : 13 – 22.
- Thomason , J.L. ; Gelbart , S.M. and Sobun , J.F. (1988) . Comparsion of four methods to detect Trichomonas vaginalis . J. Clin. Microbiol. , 26 : 1869 – 1870 .
- Yazar , S. ; Dagci , H. ; Aksoy , U. ; Ustun , S. ; Akisu , C. ; Mucide , AK. and Daldal , N. (2002). Frequency of Trichomonas vaginalis among women having vaginal discharge in Izmir , Turkey . Inonu. Univ. Tip. Fakut. Derg. , 9: 159 – 161.
- Madico , G. ; Quinn , T.C. ; Rompalo , A. ; Mckee , K.T. and Gaydos , C.A. (1998) . Diagnosis of Trichomonas vaginalis using vaginal swab sample . J. Clin. Microbiol. , 36: 3205 – 3210.
- Beaver , P. C. ; Jung , R.C. and Cupp , E.W. (1984) . Clinical parasitology . 9th ed. Lea and Febiger , Philadelphia : 825 pp.
- Sambrook , J. ; Fritsh , E.F. and Maniatis , T. (1989). Molecular cloning , alaboratory manual . 2nd ed. Cold Spring Harbor laboratory press.USA.
- Lawing , L.F. ; Hodges , S.R. and Schwebke , J.R. (2000). Detection of Trichomonas vaginalis in vaginal and urine specimens from women by culture and PCR . J. Clin. Microbiol. , 38: 3585 – 3588.
- Al-Ani ,S.F.I.H.; AI-Hadithi, I.A.W. and Al-Hadithi, R.J.K. (2001). Factors affecting vaginal trichomoniasis among women in Ramadi. J. Basrah. Res., 27 : 24- 35.
-Gani , Z.H. (2000). Urogenital trichomoniasis among women in Basrah . M. Sc. Thesis , coll. Med. Univ. Basrah , 91 pp.
- Kengne , P. ; Veas , F. ; Vidal , N. ; Rey , J. and Cuny , G. (1994). Trichomonas vaginalis : repeated DNA target for highly sensitive and specific polymerase chain reaction diagnosis . Cell. Mol. Biol. , 40 : 819 – 823.
- Wilson , S.M. (1991) . Techniques : Nucleic acid techniques and the detection of parasitic disease . Parasitol. Today. , 7: 255.
- Riley , D.E. ; Roberts , M.C. ; Takayama , T. and Krieger , J.N. (1992). Development of a polymerase chain reaction – based diagnosis of Trichomonas vaginalis . J. Clin. Microbiol. , 30: 465 – 472.
- Garcia , L.S. and Ash , L.R. (1979). Diagnostic parasitology : clinical laboratory manual. 2nd ed. , the C.V. Mosby company , London : 174 pp.
- Karyakarte , R. and Damle , A. (2003). Medical parasitology . Calcuta art studio , India : 288 pp.
- Dettovitz, J.A. ; Kelly, P ; Feldman, J. ; Sierra, M.F. ; Clark, L. and Bromberg, J. ( 1994 ) . Sexually transmitted disease , sexual behavior , and cocaine use in inner – city women . Am. J. Epidemiol., 140 : 1125 – 1134 .
- Jatau , E.D. ; Olonitola , O.S. and Olayinka , A.T. (2006) . Prevalence of Trichomonas vaginalis infection among women attending antenatal clinic in Zaira , Nigeria . Ann. Afri. Med. , 5: 178 – 181.
- Madani , T.A. ( 2006) . Sexually transmitted infection in Saudi Arabia . BMC .Infect. Dis ., 6 : 218 – 225 .
- Mahdi , N.K. ; Gani , Z.H. and Sharief , M. (2001). Risk factors for vaginal trichomoniasis among women in Basrah , Iraq. East. Med. Heal. J. , 7: 918 – 924.
- Verteramo, R. ; Calzolari, E. ; Degener, A.M. ; Masciangelo, R. and Patella , A. ( 2008 ) . Trichomonas vaginalis infection : Risk indicators among women attending for routine gynecologic examination . J. Obstet. Gynecol. Res. , 34: 233 – 237.
- Hillier , S.L. ; Willken, S.S. ; Krohn, M.A. ; Watts, D.H. ; Kiviat, N.B. and Eschenbach, D.A. ( 1993 ) . The relationship of amniotic fluid cytokines preterm delivery , amniotic fluid infection, histolytic chorioamnionitis and chorioamnion infection . Obestet. Gynaecol. , 81 : 941 – 948 .
- Sharief , M. (1998). Genital infection among women using various contraceptive methods in Basrah , Iraq . East. Med. Heal. J. , 4: 487 – 492.
