Comparative study of antibacterial effect of ethanolic leaf extracts Suaeda aegyptiaca plant and some antibiotics in the growth of pathogenic bacteria

Authors

  • Ahmed A. Naseer AL-Alamiry Cancer Research Unit , College of Medicine , Unvirsity of Thi-Qar, Iraq.

Abstract

The Antimicrobial properties of the ethanolic leaf extract of the Suaeda aegyptiaca plan, on four bacterial isolates (Stapshylococcus aureus, S.epidermidis, P.aeruginosa,and Escherichia coli),were investigated . The antimicrobial activity of the plant extract was determined by the Well diffusion method. The ethanolic leaf extract of all the plants inhibited the test organisms. The spectrum of antimicrobial activity was recorded against S. aureus(26.66-14.66 mm),S.epidermidis(22-15.22 mm), P. aeruginosa(20.33-13.33 mm), and E.coli (21.66-18.55 mm) at (10-2.5%) con. The studying extract exhibited antimicrobial properties compared favorably with some standard antibiotics .The maximum zone of inhibition of antibiotics was measured against S.aureuswas (E) (16mm), on the other hand, (ST and AM) had no effect on its.  S.epidermidis was highly sensitive to (NOR) was 38mm and resistant to (AM and L). E.coli and P.aeruginosa was highly sensitive to (AM),while it is resistant to (CAZ,PY and CXM).As well as AMC and CD had no effect on P.aerug -inosa.Compare with The screening results of the medicinal plant extract in the present study confirmed a source of antimicrobial agents by the highest sensitivity was recorded.

References

Akujobi, C., Anyanwu, B. N., Onyeze, C. and Ibekwe, V. I. (2004).Antibacterial Activities and Preliminary Phytochemical Screening of four medicinal plants.Journal of Applied Sciences.7(3); 4328 – 4338.

Alhdad, G.M., E. Charlotte, J. Mohammed, 2013.Effect of combined salinity and waterlogging on the halophyte Suaedamaritima:The role of antioxidants,Environmental and ExperimentalBotany, 87: 120–125.

Barry AL, Coyle MB, Thornsberry C, Gerland EH and Howkinson RW (1979).Methods of measuring zones of inhibition with the bauer-kirby disk susceptibility test. J. Clini. Microbiol.,10: 885-889.

Baur AW, Kirby WMM, Sherris JC, Turch M (1966). Antibiotic susceptibility testing by a standardized single disc method.Am. J. Clini. Pathol.,45: 494-496.

Bocanegra-Garcia,V.,M.Camacho-Corona,M.Ramirez-Cabrera and G.R. Garza-Gonzatez, 2009. The bioactivity of plant extracts against representative bacterial pathogens of the lower respiratory tract. BMC Research Notes, 2: 95.

Cheesbrough, M. (2001).Medical Laboratory Manual for Tropical Countries.(2ndedition). Butterworths-Heinemann. Great Britain. pp540

Cohen, M.L., 1992. Epidemiology of drug resistance: implications for a post-antimicrobial era. Science, 257(5073): 1050-1055.

Collee , J. ; Faser , A.; Marmicn , B. ; and Bimon , A. (1996) : Practical Medical Microbiology.Makie and M.C. Cartency.14th ed., Churchill Livingston.Newyork. P. 978 .

Driscoll, J.A., S.L. Brody and M.H. Kollef, 2007.The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs, 67(3): 351-368.

Esimone, C. O., Adiukwu, M. U. and Okonta, J. M. (1998).Preliminary Antimicrobial Screening of the Ethanolic Extract from the Lichen Usneasubfloridans (L). Journal of Pharmaceutical Research and Development. 3(2); 99 – 102.

Ghazanfar SA, 1994. Handbook of Arabia Medicinal Plant.Boca Raton, Ann Arbor (CRC), London, pp: 83-86 .

Heath DD, Khwaja F, Rock CL. Curcumin content of turmeric andcurry powders. FASEB J. 2004;18:A125.

Kelmanson, J.E.; Jagur, A.K.; Van Staden, J.: Zulu medicinal plants with antibacterial activity. J. Ethnophermacol, 69; 241-246, 2000.

Mann CM, Cox SD and Markham JL, The outer membrane of Pseudomonas aeruginosa NCTC 6749 contributes to its tolerance to the essential oil of Melaleucaalternifolia(tea tree oil).LettApplMicrobiol30:294–297 (2000).

Manoj M, Kailas C, Balaji V, Sajid N. Effect of Plants Extracts. International Journal of Pharm. Tech. Res. 2010; 2(1): 899-901.

Mesika, P.L.; Atolsyane, A.J.: Antimicrobial activity of some plants used for the treatment of livestock diseases in eastern cape. South Afr. J. Ethnophermacol, 83; 129-134, 2002.

National Committee for Clinical Laboratory Standards.Performance Standards for Antimicrobial Disk Susceptibility Tests.Fifth Edition. Approved Standard NCCLS Document M2-A5, Villanova, PA: NCCLS, 2002, 13(24).

Oussalah M, Caillet S, Lacroix M (2006). Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O57:H7 and Listeria monocytogenes. J. Food Protection. 69(5): 1046-1055.

Ravikumar S, Gnanadesigan M, Serebiah JS, Inbaneson SJ. Hepatoprotective effect of an Indian salt marsh herb SuaedamonaicaForrsk ex.Gmel against concanavalin-A induced toxicity in rats. Life Sci Med Res. 2010;2:1-9.

Reda, E.A., H. Saneoka, K. Fujita, 2004. Effect of salinity on osmotic adjustment glycinebetaine accumulation and the betaine aldehyde dehydrogenase gene expression in two halophytic plants Salicorniaeuropaea and Suaedamaritima, Plant Science, 166: 1345–1349.

. Torkelson AR, 1996. Cross name of index to medicinal plant.(CRC) publisher London Pp 622 .21

Wahle KW, Brown I, Rotondo D, Heys SD. Plant phenolics in the prevention and treatment of cancer. AdvExp Med Biol. 2010; 698: 36-51.

Walsh SE, Maillard JY, Russell AD, Catrenich CE, Charbonneau DL, Bartolo RG (2003). Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria. J. Appl. Microbiol. 94:240-247.

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2021-03-07

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