Evaluation of Cephalopoda extract against some nosocomial bacterial isolates

  • Al-Maliky Kh. Haydar Thi-qar university, medicine collage, microbiology department


To evaluate the pathogenicity of Staphylococcus aureus and Escherichia coli in male mice and to compare between antibacterial activity Cefotaxime  250mg and  cephalopoda extract (Sepia sp.) and showed the effect on some blood parameters in male mice. The mice were divided into seven groups (six mice for each) ,all treatments were given intraperitoneally to experimental mice. First group (control animals) were injected with 0.9 % normal saline (0.1 ml for each animal), second group were received i.p. single dose of E. coli (0.1× 108 CFU), third group were injected with a single dose of S. aureus (0.1× 108 CFU), fourth group were injected with a single dose of  E. coli (0.1× 108 CFU) then treated with (0.1 Cefotaxime  250mg) for 3 days fifth group were injected with a single dose of  S. aureus (0.1× 108 CFU) then treated with (0.1 Cefotaxime 250mg) for 3 days, sixth group were injected with a single dose of  Escherichia coli (0.1× 108 CFU) then injected with (0.1 of extract,720 µg for each animal ) for 3 days, seventh group were injected i.p. with a single dose of  S. aureus (0.1× 108 CFU) then injected with (0.1 of extract, 720 µg for each animal ) for 3 days. The results conducted that all infected mice were suffered from elevated in their body temperatures, while decline in their body weights and subsequently, changes in blood parameters compared with normal value. On the other hand, treated mice with Sepia extract show healthy and maintained their body temperatures and body weights as normal, in addition to blood parameters remained within normal ranges. These above results explained the role of Sepia extract as antimicrobial substance, acting against nosocomial bacterial isolates.


Anandia, M. and Juan Carbs, P. (2009). Procedure Manual" Mitratereductase assay " Drug susceptibility testing for Mycobacterium tuberculosis, 20:8-12.

Anderson, RS. andBeaven, AE.(2001). Antibacterial activities of oyster(Crassostreavirginica) and mussel (Mytilusedulisand Geukensiademissa) plasma.Aquat. Living Res., 14: 343-349.

Axel, K.; Ingeborg, S. and Gunter,K. (2006). How long do nosocomial pathogens persist on inanimate surfaceseven for month. These species found among the most frequent isolates from patients with nosocomial infections BMC infectious diseases, 6:2-8.

Benkendorff, K.; Bremner,J.B. and Davis, A.R. (2001). Indole derivatives from the egg masses of muricidmolluscs. Molecules, 6: 70-78.

Cryz, J.R.; Furer, E. and Germanier, R. (1983). Protection against P. auruginosa infection in a murine burn wound sepsis model by passive transfer of antitoxin A, antielastase and antilipopoly saccharide. Infect. Mune.39(3): 1072-1079.

Degaim, Z.D. (2009). Extraction of protein compound from some marine Mollusca species and study their antifungal activity. M .S . C. Thesis. Collage Science. University of Basra

Degiam, Z.D. and Abbas, A.T. (2010). Antimicrobial activity of some crude marine Mollusca extracts against some human pathogenic bacteria. Thi-Qar Medical Journal ., 4(3):142-147.

Deirdre, C.; Sameer,E.; Owen, R.; Brent, W. and Robert, L.(2006). Burn wound infection. Clinical Microbiology Reviews: 403-434.

Fournior, B. and Philpott, D. (2005). Recognition of Staphylococcus aureus by innate immune system.Clin.Microbiol. Rev., 18(3): 521-540.

Geo, F.; Brook; Janet, S. Butel and Stephen, A.M. (2004). Medical microbiology. 23ed: 252-264. Toronto,London.

Hashim, T.S. (2005).Isolation and diagnosis of burn contaminated bacteria and study the effect of fat extract on their growth. Master thesis, Baghdad.university, 10-22.

Hassan, S.H.H. (2008). Some aspect of local and systemic immunity to associated bacteria withskin burn. Ph.D.: 1-13.

Ilhan, S.; Savaroğlu, F. and Çolak, F. (2007).Antibacterial and AntifungalActivity of CorchorusolitoriusL. (Molokhia) Extracts. Inter. J. Nat.Eng. Sci., 1(3): 59-61.

Jayaraj, SS.;Thiagarajan, R.;Arumugam, M. andMullainadhan, P. (2008).Isolation, purification and characterization of [beta]-1,3-glucan bindingprotein from the plasma of marine mussel Pernaviridis. Fish ShellfishImmunol., 24: 715-725.

Litchfield, J.T and Wilcoxon F A. (1949). A simplified method of evaluating dose-effect experiments.J. Pharmacol. Exp. Ther. 96:99-113, 1949.

Lowy, FD. (1998). Staphylococcusaureusinfections.N Engl J Med 1998; 339(8): 520-532.

Manati, A.; JamshidKohanteb; DavoodMehrabanii; Aziz Japoni, MasondAmini; Ahmad Hosseia and NazaninKhalili (2009). Active immunization using exotoxin A confers protection against Pseudomonas auruginosa infection in a mouse model. BMC Microbiology. 10:9-23.

Mayer, AMS.; Rodriguez, AD.;Berlinck, RGS. andHamann, MT. (2007). Marinepharmacology in 2003-2004: marine compounds with anthelminticantibacterial, anticoagulant, antifungal, anti-inflammatory,antimalarial, antiplatelet, antiprotozoal, antituberculosis, and antiviralactivities; affecting the

cardiovascular, immune and nervous systems,and other miscellaneous mechanisms of action. Com. Biochem.

Physiol. C: Toxicol. Pharmacol., 145: 553-581.

Mitta ,G.; Hubert, F.;Dyrynda, EA.;Boudry, P. andRoch, P. (2000a). Mytilin Band MGD2, two antimicrobial peptides of marine mussels: Genestructure and expression analysis. Dev. Comp. Immunol., 24: 381-393.

Mitta, G.;Vandenbulcke,F.; Hubert ,F.;Salzet,M.and Roach, P. (2000b).Involvement of mytilins in mussel antimicrobial defense. J. Biol.Chem., 275: 12954-12962.

Nathan, A.B.; Emily, M.Z.; Paul, M.L.; Edward, A.C.; Jennifer, E.A.; Martin, L.Y. and Warren, S.H. (2000). A Model of infected burn wounds using Escherichia coli O18: K1: H7 for the study of Gram – negative bacteremia and sepsis. Infect. Immun. 68(6): 3349- 3351.

Nilsson, IM.; Patti, J.;Bremell, T.; Hook, M. andTarkowski, A.(1998).Vaccination with a recombinant fragment of collagen adhesion provides protection against Staphylococcus aureus-mediatedseptic death. J Clin Invest; 101(12): 2640-2649.

Obeidat, M., M. Shatnawi, M. Al-Alawi, E. Al-Zubi and H. Al-Dmooret al., 2012. Antimicrobial activity of crude extracts of some plant leaves. Res. J. Microbiol., 7: 59-67.

Pakrashi, A.(2001). Indian J. Physicol.Pharmacol., 45: 249-252.

Patil, R.; Jeyasekaran, G.; shanmugam, S.A.; and Jeyashakila, R.(2001). Indian J Marine.Sci 30: 264-267.

Patterson Edward, and Murugan, 2000. Indian J Marine.Sci 26:206-208.

Petra, G.; Frank, S.; Michael, B. and Christine, G. (2012). Decreasing healthcare-associated Methicillin-resistant S. aureus (MRSA) infections antibacterial resistance data from the German national nosocomial surveillance system kiss.Antimicrobial. Resist. Infect. Contral, 26:21-3.

Shanmugam,A.; Mahalakshmi,TS. andBarwin vino, A. (2008a). AntimicrobialActivity of Polysaccharides Isolated from the Cuttlebone of Sepiaaculeata (Orbingy, 1848) and Sepia brevimana (Steenstrup, 1875): An approach

to selected Antimicrobial Activity for Human pathogenicMicroorganisms. J. Fish. Aqua. Sci., 3(5): 268-274.

Shanmugam,A.;Amalraj, T. andPalpandi, C. (2008b). Antimicrobial Activity ofSulfated Mucopolysaccharides [Heparin and Heparin – LikeGlycosaminoglycans (GAGs)] from Cuttlefish EuprymnaberryiSasaki, 1929. Trends in App. Sci. Res., 3(1): 97-102.

Shigen, M.; Masaharu,Y.; Hiroshi, N. and Masaryuki, K. (2012). Experimental protection of mice against lethal Staphylococcus aureus Infection by Novel Bacteriophage.Journal of Ifectious disease. 187(4): 613-624.

Steve, K.A. and Dennis, S. (2001). Microbiology: A photographic Atlas for the laboratory. Benjamin Cummings. An imprint of Addison Wesley Longman, Inc., 105-108.

Vairamani,S.; Subhapradha,N.; Ramasamy,P.; BarwinVino,A.; Raveendran, S. and Shanmugam, A. (2012). Antibacterial Activity of Methanolic Extract of Whole Body

Tissue and Ethylene Diamine Tetra Acetate Extract of Cuttlebone of Sepiellainermis(Orbigny, 1848).Research Journal of Microbiology, 7: 263-272.

Victor, L.Y.; Dennis, S. H.; Hansen, T.; Wen, C.K.; Keith, p.K. and Anne, V.G.(2007). Virulence Characteristics ofKlebsiella and Clinical Manifestations of K. pneumoniae Bloodstream Infections.Emerging infectious diseases. 13(7): 986-992.

Worms, J.(1983). World fisheries for cephalopods.A synoptic overview.In: Caddy, JF (Eds), Advances in Assessment of World CephalopodResources. FAO Fish, Tech. Paper, pp. 231- 452.