Contamination and Microbial Biofilm Formation in intensive care unit in Thi-qar province / Iraq
Abstract
To identify the cause and the source of bacterial infection among patients of intensive care unit and to identify the ability of bacteria to produce biomass this study had been conducted in Al-Imam Al-Hussein hospital in Thi-qar province for the period from 1st September to end of December 2011 .A total of 320 swabs and samples were collected from 17 different sites of Intensive Care Unit environment and inoculated on a normal cultural media ,then incubated at 37°C for 24 hour . The growth revealed different bacterial colonies which had been tested for their morphological and biochemical characteristics. Sixty eight of pure isolates were obtained including 24 (35.29%) Gram positive bacterial isolates, 44(64.71%) of Gram negative bacterial isolates , the highest rates (19.11%) of bacterial contamination had been found on the walls and the floor . Sensitivity tests for all isolates were done using 25 types of commonly used antibiotics in Iraq , the results revealed that the genus Enterobacter spp. had a high resistance as a Gram negative bacteria , and Staphylococcus spp. had a high resistance as a Gram positive bacteria to most of the tested antibiotics, The tendency of some isolates to develop a biomass as biofilm , an important virulence determinant related to infection , was investigated in vitro using microtiter plates . The highest optical density (O.D.= 0.634 n.m.) was recorded by the isolate from pressure material by Pseudomonas aeruginosa and the least O.D. was recorded by medical instruments-use manual isolate (O.D. was 0.106 n.m.) by Pantoea spp .References
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