EVALUATION OF SEMINAL FLUID PARAMETERS AFTER IN VITRO SPERM PREPARATION TECHNIQUE IN NON VARICOCELIC AND VARICOCELIC INFERTILE MEN UNDERGOING VARICOCELECTOMY
Keywords:Male infertility, Varicocele, Antisperm antibody, Varicocelectomy
AbstractThis study was designed to evaluate and compare the results of semen parameters, sperm membrane potential integrity and viability for non varicocelic and varicocelic infertile men after varicocelectomy. Thirty semen samples (varicocelic) and twenty normozoospermic men (non varicocelic) as a control group were collected by masturbation in the special semen room collection and prepared by simple layering technique. The semen samples were analyzed and prepared by standard semen parameters. Furthermore, sperm concentration, sperm motility, progressive sperm motility, sperm agglutination, normal sperm morphology, sperm HOST, and sperm Eosin stain were evaluated according to standard WHO criteria (1999). However, direct immunobead assay was used to determine the presence of AS-AB bound on sperm surface. For preparation technique, sperm prepared and incubated for 30 minute in 5% CO2 at 37ºC after in vitro sperm processing. The results of the present study indicate a highly significant (P<0.001) differences in all sperm functions parameters of varicocelic infertile men in AS-ABs positive (HOST and Eosin stain; negative) as compare with that noticed in normozoospermic men in AS-AB negative (HOST and Eosin stain; positive). It was concluded that there are a strong positive correlation between varicocele and antisperm antibodies generation and between antisperm antibodies and semen characteristics and HOST-Vitality test. Further studies are recommended to assess the detrimental effect of AS-ABs on DNA damage and embryo quality after in vitro fertilization and embryo transfer in assisted reproductive technologies (IVF-ET-ART).
Chamley LW. and Clarke GN. (2007): Antisperm antibodies and conception. Semen Immunopathol. 29: 169-84.
Vujisić S., Lepej SZ. and Jerković L. (2005): Antisperm antibodies in semen, sera and follicular fluids of infertile patients: relation to reproductive outcome after in vitro fertilization. Am. J. Reprod. Immunol. 54: 13-20.
Chiu WW. and Chamley LW. (2004): Clinical associations and mechanisms of action of antisperm antibodies. Fertil. Steril. 82: 529-35.
Heidenreich A., Bonfig R. and Wilbert DM. (1994): Risk factors for antisperm antibodies in infertile men. Am. J. Reprod. Immunol. 31: 69-76.
Collins JA., Burrows EA., Yeo J. and Young Lai EV. (1993): Frequency and predictive value of antisperm antibodies among infertile couples. Hum. Reprod. 8: 592-8.
Omu AE., Makhseed M., Mohammed AT. and Munim RA. (1997): Characteristics of men and women with circulating antisperm antibodies in a combined infertility clinic in Kuwait. Arch. Androl. 39: 55-64.
Nagy ZP., Verheyen G. and Liu., J. (1995): Results of 55 lntracytoplasmic sperm injection cycles m the treatment of male-immunological infertility. Hum. Reprod. 10: 1775-1780.
Pagidas K., Hemmings R., Falcone T. and and Miron P. (1994): The effect of antisperm autoantibodies in male or female partners undergoing in-vitro fertilization-embryo transfer. Fertil. Steril. 62: 363-369.
Lahteenmaki A., Reima I. and Hovatt O. (1995): Treatment of severe male unmunological infertility by lntracytoplasmic sperm injection. Hum. Reprod. 11: 2824-2828.
Marshbura PB. and Kutteli WH. (1994): The role of antisperm antibodies in infertility. Fertil. Steril. 61: 799-811.
Said SA., Aribarg A. and Virutamsen P. (1992): The influence of varicocele on parameters of fertility in a large group of men presenting to infertility clinics. Fertility and Sterility. Vol. 57, No. 6, Pp. 1289-1293.
Ficarra V., Porcaro AB., Righetti R. (2002): Antegrade scrotal sclerotherapy in the treatment of varicocele: a prospective study. British Journal of Urology International, Vol. 89, No. 3, Pp. 264-268.
Golomb J., Vardinon N., and Homonnai ZT. (1986): Demonstration of antispermatozoal antibodies in varicocele-related infertility with an enzyme-linked immunosorbent assays (ELISA). Fertility and Sterility. Vol. 45, No. 3, Pp. 397-402.
Pasqualotto FF., Sundaram A., Sharma RK., Borges E., Pasqualotto EB. and Agarwal A. (2008): Semen quality and oxidative stress scores in fertile and infertile patients with varicocele. Fertility and Sterility. Vol. 89, No. 3, Pp. 602-607.
Kiran R. and Shrivastav TG. (2007): A correlation study between steroid hormone levels and anti-sperm antibodies in serum and seminal plasma of men with or without reduced sperm motility,” Journal of Endocrinology and Reproduction. Vol. 1, Pp. 31-35.
Hjort T. and Hansen KB. (1971): lmmunofluorescent studies on human spermatozoa. Clin. Exp. Immunol. 8: 9
Rehan NE., Sabrero A J. and Fertig JW. (1975): The semen of fertile men. Statistical analysis of 1200 men. Fertil. Steril. 26: 492.
Amelar RD. and Dubin D. (1977): Semen analysis. In: Amelar RD., Dubin D P. and Walsh C. (eds): Male Infertility. W. B. Saunders Co., Philadelphia., Pp. 105-141.
MacLeod J. (1976): Seminal cytology in the presence of varicocele.Fertil.Steril.27: 110.
Quesada EM., Duken CD. and Deen GH. (1986): Genital infection and sperm agglutinating antibodies in infertile men. J. Urol: 99. 106.
West D., Chehval MJ., Winkelmann T. and Martin SA. (2000): Effect of vasovasostomy on contralateral testicular damage associated with unilateral vasectomy in mature and immature Lewis rats. Fertil. Steril. 73:238-241.
Matsuda T., Horii Y. and Yoshida O. (1992): Unilateral obstruction of the vas deferens caused by childhood inguinal herniorrhaphy in male infertility patients. Fertil. Steril. 58:609-613.
Flickinger CJ., Vagnetti M., Howards SS. and Herr JC. (2000): Antisperm autoantibody response is reduced by early repair of a severed vas deferens in the juvenile rat. Fertil. Steril. 73:229-237.
Chehval MJ., Martin SA., Alexander NJ. and Winkelmann T. (1995): The effect of unilateral injury to the vas deferens on the contralateral testis in immature and adult rats. J. Urol. 153:1313-1315.
Haas GG. (1987): Antibody-mediated causes of the male infertility. Urol. Clin. North. Am. 14:539.
Setchell BP., Voglamyer JK. and Waites GM. (1969): A blood-testis barrier restricting passage from blood rete testis fluid but not into lymph. J. Physio. 200:73-85.
Sainio-Pollanen S., Saari T., Simell O. and Pollanen P. (1996): CD28-CD80/CD86 interactions in testicular immunoregulation. J. Reprod. Immunol. 31:145-163.
Sakamoto Y., Matsumoto T., Mizunoe Y., Haraoka M., Sakumoto M. and Kumazawa J. (1995): Testicular injury induces cell-mediated autoimmune response to testis. J. Urol. 153:1316-1320.
Almagor M., Dan-Goor M., Hovav Y. and Kafka I. (1998): Antisperm antibodies in men with psychogenic anejaculation. Arch. Androl. 41:1-4.
Shibahara H., Tsunoda T., Taneichi A., Hirano Y., Ohno A., Takamizawa S., Yamaguchi C., Tsunoda H. and Sato I. (2002): Diversity of antisperm antibodies bound to sperm surface in male immunological infertility. Am. J. Reprod. Immunol. 47:146-150.
Bubanovic I. (2004): 1, 25-dihydroxyvitamin D3 as New Immunotherapy in Treatment of Recurrent Spontaneous Abortion. Med Hypotheses 63:250-253.
Zavos PM. and Centola GM. (1990): Qualitative and quantitative improvements in human spermatozoa recovered via swim-up and a new semen filtration method. Infertility. 57:1326-30.
Correa JR. and Zavos PM. (1996): Preparation and recovery of frozen-thawed bovine spermatozoa via various sperm selection techniques employed in assisted reproductive technologies. Theriogenology. 46:1225-32.
Zavos PM., Correa JR., Sofikitis N., Toda T. and Zarmakoupis-Zavos PN. (1996): The usefulness of pentoxifylline for the recovery of human spermatozoa in assisted reproductive technologies. Middle East Fertil. Soc. J. 1:128-33.
Clarke GN., Lopata A. and Johnston WIH. (1984): Effect of sperm antibodies in females on human in vitro fertilization. Fertil. Steril. 46:438.
HAAS GG. (1992): Male infertility and immunity In: Infertility in Male. Lipshultz L. and Howards SS. (eds). Mosby Yem Book, St Louis, Pp: 277.
GOLOMB J., VARDINON N. and HOMO1 NAI Z T. (1997): Demonstration of antispermal zoal antibodies in varicoceIe related infertility with an enzyme-linked iummunosorbe assay (ELISA). Fertil. Steril. 45:397.
World Health Organization (WHO) (1999): Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, 4th ed. Cambridge, Cambridge University Press UK. Pp.8-11.
Yanagimachi R., Yanagimachi H. and Rogers B. (1976): The use of zona-free animal ova as a test system for the assessment of the fertilizing capacity of human spermatozoa. Bio. Reprod. 15:471-6.
Yandav SB., Sardeshamukh AS. and Suryakar AN. (2001): A study of seminal hyaluronidase, fructose, lipid peroxide and Zinc in primary male infertility. J.Obst.Gyn. 51: 142-145.