• Manal Mohammed Kadhim Lecturer of Medical Microbiology/ College of Medicine/ Al.Qadisiah University
  • Jaafar Kadhim AlMousawi Assistant Professor of Clinical Immunology/ College of Medicine/ Al-Kufa University


        Neonatal sepsis is a major  cause of death in  newborns despite sophisticated neonatal intensive care. This cross-section study was done on 69 neonates with suspected sepsis who were admitted in Neonatal Intensive Care Unit (NICU) of Maternity and Child Teaching Hospital at AL-Diwaniya city, and 20 healthy neonates as a (control group) in the period from March to October 2009.This study was conducted to evaluate the diagnostic value of polymerase chain reaction (PCR) for bacterial DNA component encoding 16S rRNA in the early diagnosis of neonatal sepsis prior to the blood culture ( the golden standard test). The investigation protocol included blood culture and 1 mL of venous blood for molecular analysis by polymerase chain reaction (PCR) for bacterial DNA component encoding 16 s RNA in all cases, We compared the results of PCR with blood culture. The culture positivity rate 20 (28.9%) among suspected sepsis neonates, with sensitivity of (29%)and specificity of(100%).The male more affected than female among proven sepsis with ratio 3:2. It was found a high sensitivity (100%), high specificity (87.5%) and positive predictive value of (98.6), negative predictive value of (100%)for PCR analysis for bacterial DNA component encoding 16S rRNA .Blood culture is the most reliable method for diagnosis of neonatal sepsis. Polymerase chain reaction is useful and superior to blood culture for early diagnosis of sepsis in neonates.


- Gaini,S.; Koldkjaer,O.G.; and Pedersen,C.;(2006):"Procalcitonin, lipopolysaccharide -binding protein, interleukin-6 and C-reactive protein in community acquired infections and sepsis: a prospective study. Crit Care ;10(2):53.

- Mandell, G.L; Bennett, J.E; and Dolin, R.(2005): Principles and Practice of Infectious Diseases. 6th ed. London: Churchill Livingstone.

- Magudumana, M.O.; Ballot, D.E.;and Cooper, P.A.;(2000):"Serial interleukin 6 measurements in the early diagnosis of neonatal sepsis". J Trop Pediatr ;46(5):267-71.

- Clarridge, J. E.; (2004):" Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases". Clin. Microbiol. Rev. 17(5):840–862.

- van Haeften, R.; Palladino S.; and Kay,I.; (2003):"A quantitative LightCycler PCR to detect Streptococcus pneumoniae in blood and CSF". Diagn. Microbiol. Infect. Dis. 47:407–414.

- Nikkari, S., Lopez, F. A. ;and Lepp, P. W. ;( 2002):"Broad-range bacterial detection and the analysis of unexplained death and critical illness". Emerg. Infect. Dis. 8:188–194.

- Klaschik, S.; Lehmann, L. E.; and Raadts,A.;(2004):"Detection and differentiation of in vitro-spiked bacteria by real-time PCR and melting-curve analysis". J. Clin. Microbiol. 42:512– 517.

- Vandepitte,J.;(2003):Basic Laboratory Procedures in clinical bacteriology, 2nd ed. Geneva; WHO.

- Kocabas, E.;A. Sarikcioglu, A.;and N. Aksaray,N.;(2007): “Role of procalcitonin, C-reactive protein, interleukin-6, interleukin-8 and tumor necrosis factor-α in the diagnosis of neonatal sepsis,” Turkish Journal of Pediatrics, 49(1): 7–20.

- Manucha, V.; Rusia, U.; and Faridi, M. M. A.;(2002).“Utility of hematological parameters and C-reactive protein in the detection of neonatal sepsis” Journal of Pediatrics and Child Health, 38(5): 459–464.

- Shaw,C.K.; Shaw, P.; and Thapalial,A.;(2007):" Neonatal sepsis bacterial isolates and antibiotic susceptibility patterns at a NICU in a tertiary care hospital in western Nepal: A retrospective analysis. Kathmandu University Medical Journal; 5(4): 153-160.

- Buttery, J.P.;(2002):"Blood cultures in newborn and children: optimizing an everyday test". Arch Dis Child Fetal Neonatal Ed ;87(6):25-7.

- Ananthakrishnan ,S.; and Gunasekaran, D.;(2009):"Etiology and risk factors for early onset neonatal sepsis". Indian J Med Microbiol ; 30;27:279.

- Sourabh D.;et. al.,(2009):"Diagnosis of Neonatal Sepsis Using Universal Primer Polymerase Chain Reaction Before and After Starting Antibiotic Drug Therapy".Arch Pediatr Adolesc Med.;163(1):6-11.

- Borna,H.; and Borna, S.;(2005):"Value Of Laboratory Tests And C- Reactive Protein In The Detection Of Neonatal Sepsis".The Internet Journal of Pediatrics and Neonatology; 5 (2):23.

- Ziba,M.;Amir,H.M.;and Reznan,M.,(2003):"Profile of Bacterial Sepsis in Neonates from Kashan in Iran'.J Infect Dis Antimicrob. Agents;20:97-102.

- Cordero,L.; Rau, R.; Tylor,D.;(2004):"Enteric Gram-negative bacilli bloodstream infections:17 years, experience in a neonatal intensive care unit".Am.J.Infect. Control;32(80):189-195.

- Ashok, K.; Yadav, C.G.; and Menon P.K.;(2005): "Polymerase Chain Reaction in Rapid Diagnosis of Neonatal Sepsis". Indian J Pediatr; 42:681-685.

- Tonje, R.N.;(2009):"Comparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: a case control study". BMC Pediatrics, 9(1):5.