THE ROLE OF PCR AMPLIFICATION OF 16S RRNA IN EARLY DIAGNOSIS OF NEONATAL SEPSIS
Abstract
Neonatal sepsis is a major cause of death in newborns despite sophisticated neonatal intensive care. This cross-section study was done on 69 neonates with suspected sepsis who were admitted in Neonatal Intensive Care Unit (NICU) of Maternity and Child Teaching Hospital at AL-Diwaniya city, and 20 healthy neonates as a (control group) in the period from March to October 2009.This study was conducted to evaluate the diagnostic value of polymerase chain reaction (PCR) for bacterial DNA component encoding 16S rRNA in the early diagnosis of neonatal sepsis prior to the blood culture ( the golden standard test). The investigation protocol included blood culture and 1 mL of venous blood for molecular analysis by polymerase chain reaction (PCR) for bacterial DNA component encoding 16 s RNA in all cases, We compared the results of PCR with blood culture. The culture positivity rate 20 (28.9%) among suspected sepsis neonates, with sensitivity of (29%)and specificity of(100%).The male more affected than female among proven sepsis with ratio 3:2. It was found a high sensitivity (100%), high specificity (87.5%) and positive predictive value of (98.6), negative predictive value of (100%)for PCR analysis for bacterial DNA component encoding 16S rRNA .Blood culture is the most reliable method for diagnosis of neonatal sepsis. Polymerase chain reaction is useful and superior to blood culture for early diagnosis of sepsis in neonates.References
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