STUDY OF OXIDANT-ANTIOXIDANT STATUS IN RECURRENT SPONTANEOUS ABORTION
AbstractObjective: To evaluate the association of oxidative stress and impaired antioxidant defense system with the occurrence of recurrent spontaneous abortion (RSA) . Study design : Case –control study consisting of 100 pregnant women with a history of RSA (study group) and 100 healthy pregnant women (control group) , their age ranging (15-45yr.) and both groups are classified into many subgroups according to maternal age , gestational age , parity and number of previous abortions . Fasting blood samples were collected and tested for measurement of serum vitamin C , vitamin E , albumin , oxidative stress biomarker malondialdehyde (MDA) and some essential trace elements (zinc and copper) by using spectrophotometric methods .Student t-test , chi-square and correlation analysis were used for statistical analysis . Results : Serum level of vitamin C , vitamin E , Cu and Zn were significantly lower (p<0.05) and serum level of MDA was significantly higher (p<0.05) in study group as compared with control group but no significant difference of serum albumin (p>0.05) between the two studied groups .Also no significant differences (p>0.05) of all measured biochemical parameters among different age , gestational age, parity and no. of abortions subgroups of patients (study group) except serum albumin, Zinc and Cu/Zn ratio were significantly changed in the last parity subgroup (>P5) as compared with (P1) for albumin level and with other parity subgroups of the same study group for Zinc and Cu/Zn ratio . While there was a significant increase (P<0.05) of MDA level in the (≥7 abortions) as compared with other no. of abortions subgroups of the study group . The linear regression analysis demonstrated a significant ( r=0.61 , 0.85 , 0.3 , 0.73 , 0.47 , p<0.05) negative correlation for vit.C , vit. E , Zn , Cu and Cu/Zn ratio respectively and significant ( r= 0.83 , p<0.05 ) positive correlation for MDA values with no. of previous abortions of study group . Also there is a significant ( r= 0.18 , 0.19 , 0.25 , 0.2 , p<0.05 ) negative correlation for vit.C , vit.E , Cu and Cu/Zn ratio respectively and significant ( r= 0.2 , p<0.05 ) positive correlation for MDA values with the age of pregnant females of both control and study groups . while there is a significant positive correlation (r=0.16, 0.18, 0.19 , p<0.05) of serum vit.C , vit.E and Zn respectively and a significant negative correlation (r=0.17, p<0.05 ) of serum MDA with parity in both control and study groups . Conclusions : Our data demonstrate that elevation of lipid peroxides (MDA) together with impaired antioxidant defense and status of trace elements and the presence of possible interrelationship between those parameters may be related at least partly to the pathogenesis of RSA .
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