EFFECT OF THE STORAGE ON THE AVTIVITIES OF THE ENZYMES CREATINE KINASE AND LACTATE DEHYDROGENASE IN SERUM AND BLOOD

Abstract

Objective: This study was carried out to investigate the effect of storage on the activities of the enzymes creatine kinase (CK) and lactate dehydrogenase(LDH).For this purpose we measured the levels of CK and LDH  activities in the blood and serum samples ,stored at 4C° ,during 0 time, and 24 hour. Method: Total of 51 subjects (32 males and 19 females),mean age was 20 years (range:18-22years) were selected for the study. They were non-smoker, apparently ,healthy persons, their weights within normal range, and they had no family history of diseases and no drug had been taken in last week. Blood samples were collected after an average fasting for 12 hours, and  the level of the enzymes creatine kinase (CK) and lactate dehydrogenase(LDH)  were   determined immediately (i.e the zero time) and then later after 24 hours .All samples were stored at 4C°. Results: In serum sample and during 24 hours of storage ,at 4C° the  levels of the CK and LDH enzymes  approximately remained constant. In plasma, analysis was made immediately after blood collection and after 24 hours . ASignificant increased in the levels of CK and LDH enzymes activities . Conclusion: Based on these results, we can conclude that a contact between blood cells and plasma stored at 4C° for an overnight ,my produce changes in the activities of CK and LDH enzymes.