EFFECT OF THE STORAGE ON THE AVTIVITIES OF THE ENZYMES CREATINE KINASE AND LACTATE DEHYDROGENASE IN SERUM AND BLOOD
Keywords:Storage, blood, serum, Enzymes activities
AbstractObjective: This study was carried out to investigate the effect of storage on the activities of the enzymes creatine kinase (CK) and lactate dehydrogenase(LDH).For this purpose we measured the levels of CK and LDH activities in the blood and serum samples ,stored at 4C° ,during 0 time, and 24 hour. Method: Total of 51 subjects (32 males and 19 females),mean age was 20 years (range:18-22years) were selected for the study. They were non-smoker, apparently ,healthy persons, their weights within normal range, and they had no family history of diseases and no drug had been taken in last week. Blood samples were collected after an average fasting for 12 hours, and the level of the enzymes creatine kinase (CK) and lactate dehydrogenase(LDH) were determined immediately (i.e the zero time) and then later after 24 hours .All samples were stored at 4C°. Results: In serum sample and during 24 hours of storage ,at 4C° the levels of the CK and LDH enzymes approximately remained constant. In plasma, analysis was made immediately after blood collection and after 24 hours . ASignificant increased in the levels of CK and LDH enzymes activities . Conclusion: Based on these results, we can conclude that a contact between blood cells and plasma stored at 4C° for an overnight ,my produce changes in the activities of CK and LDH enzymes.
-Zhang DJ, Elswick RK, Miller WG, Bailey JL. Effect of serum-clot contact time on clinical chemistry laboratory results. Clin Chem. 44:1325-1333. 1998.
-A. Marjani : Effect Of Storage Time And Temperature On Some Serum Analytes . The Internet Journal of Laboratory Medicine. 2(2).2007
-Rehak NN, Chiang BT. Storage of whole blood: effect of temperature on the measured concentration of analytes in serum. Clin Chem. 34:2111-2114.1988
-Young DS, Bermes EW. Specimen collection and processing: sources of biological variation. In : Burtis CA, Ash wood ER(eds). Tietz textbook of clinical chemistry. Philadelphia: WB saunders company, ,pp.42-72.1999
-NCCLS Infobase 95, H18-A, Procedures for the handling and processing of blood specimens; approved guideline. Villanova, PA: NCCLS, 1995..
_Lippi.Giuseppe;Blanckaert.Norbert;Bonini.Pierangelo;Green.Sol;Kitchen.Steven;Palicka.Vladimir;etal.Haemolysis :an over view of the leading cause of un suitable specimens in clinical laboratories.Clinical chemistry and laboratory medicine.volume 46(6):764-772.2008
-. Lippi G, Salvagno LG, Montagnana M, Brocco G, Guidi GC. Influence of hemolysis on routine clinical chemistry testing. Clin Chem Lab Med;44:311-6.2006
- Thomas L. Haemolysis as influence and interference factor. eJIFCC vol 13 no 4: Available at: http://www.ifcc.org/ejifcc/vol13no4/130401002.htm. Acessed: September 25, 2010.
- Boyanton BL, Jr, Blick KE. Stability studies of 24analytes in human plasma and serum. Clin Chem 48:2242–2247.2002 [PubMed]
-. O'Keane MP, Cunningham SK. Evaluation of three different specimen types (serum, plasma lithium heparin, and serum gel separator) for analysis of certain analytes: clinical significance of differences in results and efficiency in use. Clin Chem Lab Med 44:662–668.2006 [PubMed]
-. Rossing RG, Foster DM. The stability of clinical chemistry specimens during refrigerated storage for 24 hours. Am J Clin Pathol 73:91–95.1980 [PubMed]
-Monden S, Tanaka M, Takahashi H. Pre-test blood samples at clinical laboratories--effects of temperature and duration of blood sample storage on measurements. Rinsho Byori. Mar;56(3):239-42.2008.
-HeilnsM, Heil W, WitholdW.Storage of serum or whole blood samples,Effects of time and temperature on 22serum analytes.Eur.J.Clinical chem.Biochem.33(4):231-238.1995
-Tai-chu Peng, phd;Bang-Gee Hsu,PhD,Fwu-Lin Yang,MD,PhD,Yann FenC,.Chao,PhD,Horng-Jyh Harn,MD,PhD,and Ru-Ping Lee,RN,PhD.Stability of Blood Biochemistry levels in Animal Model Research:Effects of StorageCondition and Time.Biological Research for Nursing.11(4):395-400;2010
-Clausen T. Everts ME .Regulation of Na-K pump in skeletal muscle.Kidney int 35:1-13.1989.
-16.Banf,G,Kitta;Bauer,K,Klahr,D,Brand,W,Kolpe,D,Buchberger,M,KuKuK,Deom,A,Kunert,T,Ehret,W,Lammer,M;etal.Use of laboratory investigation and stability of blood ,plasma and serum samples.World HeathOrganisation,Geven.http://Whqlibdoc.Who.int/hq/2002/WHO-DIL-LAB-99,1-Rev.2.pdf.
-RPGoodrich,SO sowemimo-Coker,CRZerez,and KR Tanaka.Preservation of metabolic activity in lyophilized human erythrocytes.Proc Natt Acad Sciu U.S.A.89(3:967-971.1992)