• Ahmed A.Naser Al-Amiry College of medicine ,dep.of physiology,University of Thi-Qar
  • Hiba abdul-Atheem College of medicine ,dep.of Biochemistry,University of Thi-Qar.
  • Moayed Naji Majeed College of medicine ,dep.of pediatrics,University of Thi-Qar


The aim of the current study was to compare antioxidant defenses and oxidative stress markers in patients with Diabetes Mellitus (DM) and in those with Rheumatoid Arthritis (RA). Across-sectional study was conducted with 74 participants in two distinct groups: Diabetes Mellitus types 2, Rheumatoid Arthritis and healthy blood donors. Malondialdehyde and uric acid were compared between these groups. Uric acid was measured as antioxidant defenses tested in blood samples from (RA) and (DM), rather than the degree of lipid peroxidation was measured in term of malondialdehyde (MDA). MDA level was 3.29 ± 0.59 µmol/L in control group which was significantly lower than in diabetic and Rheumatoid arthritis patients 6.32 ± 0.84 µmol/L , 6.75 ± 0.77 µmol/L respectively with (p <0.001). Moreover; concentrations of UA in DM was 3.65 ± 0.11 mg/dl significantly lower than in control 4.56 ± 0.29 mg/dl with (p <0.001),conversely, we found that no significant change in the level of UA in RA 4.23 ± 0.66 mg/dl, compared with health subjects 4.56 ± 0.28 mg/dl. These results suggest that the feasibility to find out a new evidences for a possible relationship between these pathologies can be found.


Salem M. Kholoussi S.Κholoussi N.Fawzy R.Malondialdehyde and trace element levels in patients with type 2 diabetes mellitus.Archives of hellenic med. 2011;28(1):83-88.

Nakhjavani M, Esteghamati A, Nowroozi S, Asgarani F, Rashidi A, Khalilzadeh O.Type 2 diabetes mellitus duration: an independent predictor of serum malondialdehyde levels.Singapore Med J 2010; 51(7) : 582-585.

Slatter D.A. Bolton C.H. Bailey A.J.The importance of lipid-derived malondialdehyde in diabetes mellitus. Diabetologia 2000; 43: 550-557.

SuryawanshiN.P. BhuteyA.K. NagdeoteA.N. JadhavA.A. of lipid peroxide and lipid profile in diabetes mellitus. Indian J of ClinlBiochemy 2006; 21 (1) :126-130.

Galli, F.; Piroddi, M.; Annetti, C.; Aisa, C.; Floridi, E.; Floridi, A. Oxidative stress and reactive oxygen species. Contrib. Nephrol., 2005;149: 240-260.

Gambhir, J. K.; Lali, P.; Jain, A. K. Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis.Clin.Biochem.,1997; 30(4): 351-355.

Hitchon, C. A.; El Gabalawy, H. S. Oxidation in rheumatoid arthritis. Arthritis Res. Ther., 2004;6(6),:265-278.

Kamanli, A.; Naziroglu, M.; Aydilek, N.; Hacievliyagil, C. Plasma lipid peroxidation and antioxidant levels in patients with rheumatoid arthritis. Cell Biochem.Funct.,2004;22(1): 53-57.

Shaabani, Y.; Foroughi, M; Rastmanesh, R; Jamshidi, A; Tajik, N; Assadi, O. Assessment of antioxidant nutrient intake and Malondiadehyde plasma level in rheumatoid arthritis. ARYA Atherosclerosis J.2009; 5(1): 1-5.

Quinn L. Behavior and biology: The prevention of type 2 diabetes. J CardiovascNurs 2003;18:62−68.

Mahreen R, Mohsin M, Nasreen Z, Siraj M, Ishaq M. Significantly increased levels of serum malonaldehyde in type 2 diabetics with myocardial infarction. Int J Diabetes DevCtries 2010;30:49−51.

Panasenko, O. M.; Vol'nova, T. V.; Azizova, O. A.; Vladimirov, Y. A. Free radical modification of lipoproteins and cholesterol accumulation in cells upon atherosclerosis. Free Radic. Biol. Med., 1991,;10(2):137-148.

Scott DL, Symmons DP, Coulton BL, PopertAJ. Long-term outcome of treating rheumatoid arthritis: results after 20 years. Lancet 1987;1:1108–11.

Mitchell DM, Spitz PW, Young DY, Bloch DA, McShane DJ, Fries JF. Survival, prognosis,and causes of death in rheumatoid arthritis.Arthritis Rheum1986;29:706–14.

Pincus T, Callahan LF, Sale WG, Brooks AL, Payne LE, Vaughn WK. Severe functional declines, work disability, and increased mortality in seventy-five rheumatoid arthritis patients studied over nine years. Arthritis Rheum 1984;27:864–72.

Isomaki H. Long-term outcome of rheumatoid arthritis. Scand J Rheumatol Suppl 1992;95:3–8.

Wolfe F. The natural history of rheumatoid arthritis. J Rheumatol Suppl 1996;44:13–22

Paredes, S.; Girona, J.; Hurt-Camejo, E.; Vallve, J. C.; Olive, S.; Heras, M.; Benito, P.; Masana, L. Antioxidant vitamins and lipid peroxidation in patients with rheumatoid arthritis: Association with inflammatory markers. J. Rheumatol., 2002; 29(11):2271-2277.

Joan H. P. Robert B.Z. David A.L.Analysis of the thiol status of peripheral blood leukocytes in rheumatoid arthritis patients. J Leukocyte Biol.2007;81:1-8.

Satoh, K. Serum lipid peroxide in cerebrovascular disorder determined by new colorimetric method. Clin.Chim.Acta 1978;90,:37-43.

WULF, D. Free radicals in the physiological control of cell function. Physiol Rev.2002;82: 47–95.

jain SK:Hyperglycemia can causes membrane lipid peroxidation and asmotic fragility in human red blood cells.J.Boil.Chem.1989;264:21340-21345.

Wierusz-Wysooka B,Wysocki H,Wyks H:Metabolic control aulity and free radicals activity in diabetic patients.Diabetetes res.Clin.Pract.1995;72. 193-207.

Nevi S,Bruno CM,Raciti C and Angelo B:altration of oxide reductive and harmostic factor in type 2diabetes J.Inter.Med.,1994;236(5):495-500.

Sarban, S.; Kocyigit, A.; Yazar, M.; Isikan, U. E. Plasma total antioxidant capacity, lipid peroxidation, and erythrocyte antioxidant enzyme activities in patients

Cotraneo P,Manto A,Todaro L,and Magnant p:Hyper filtration in patient with diabetes mellitus:a prevalence study:Clin Nephrol.1998;50:214-217.

Erdberg A,boner G,Van Dyck DJ and Care R:Uric acid excretion in patient with non-insulin dependent diabetes mellitus nephron,1992;60:134-137.

Gonzalez-Sicilia L,Garcia-Estan J,Martinez-Blazguez A and Frnandez-pardo J:Renal metabolism of uric acid in type 1 insulin dependent diabetic patient:Horm.Meta.Res.1997;29:520-523.

Sinclair AJ,Lunec,Girling AJ and Barnett AH:Modulatores of free radical activity in diabetes mellitus:Rol of ascorbic acid.EXS.1992;62:342-352.