USE ELISA TECHNIQUE TO DETECT VIRAL HEPATITIS IN THI-QAR PROVINCE : A RETROSPECTIVE STUDY
AbstractThis study reports the prevalence of hepatitis A Virus (HAV), hepatitis B virus (HBV) and C virus (HCV), and the frequency of potential exposure to these viruses among patients were admitted to Hormones laboratory in Al-Hussain teaching hospital /Thi-Qar province. Serum samples were tested for HAV , HBV markers and antibodies to HCV by enzyme-linked immunosorbent assay (ELISA). From the results of the present study , 2775 patients were tested, divided into tow groups. Group A included the catheter patient and other group (Group B) included outpatient. Results showed no positive result for anti- HAV. While, Anti-HBs were positive 4 cases and 6 cases for group A and group B respectively. Contrast, anti- HCV were positive 2 cases for group A and 5 cases for group B. Statistically significant observation P<0.05 between the two groups, Group B was the highest sensitive to Hepatitis B,C infection than group A . Background: Diagnosis is a vital decision point at which adequate evidence has to be accumulated to get a clear clinical picture about the patient before initiation of the treatment . Laboratory tests for hepatitis are very critical for the physician to confirm his findings about the clinical condition of the patient because of several causative agents of the disease. Hepatitis being caused by the virus A,B,C diagnosis is even more complicated as all of them present similar symptoms. Therefore, laboratory testing is important to identify the exact causative virus so that the appropriate treatment can be initiated accordingly.
Jorgensen,C.( 2010). National Center for Chronic Disease Prevention and Health Promotion Centers for Disease Control and Prevention . U.S. Department of Health and Human Services, Office on Women’s. Frequently Asked Questions , 1- 4 .
Centers for Disease Control and Prevention (CDC)(2002a).Medical management of chronic hepatitis B and chronic hepatitis C . 2002;1-3.www.CDC/gov/idu I D U. H I V .Prevention
Centers for Disease Control and Prevention (CDC)(2002b).Sexually transmitted diseases treatment guidelines. MMWR Recomm Rep. 2002;51(RR-6):1-78.
•Centers for Disease Control and Prevention( 2009). Hepatitis C FAQs for the Public.1600 Clifton Rd. Atlanta, GA 30333, USA
Dienstag, J. L. (2008). "Hepatitis B virus infection" (Free full text). The New England journal of medicine 359 (14): 1486–1500.
Eurasian Harm Reduction Network. (2007). Hepatitis C among Injecting Drug Users in the New EU Member States and Neighboring Countries. Situation, Guidelines and Recommendations. Vilnius: Eurasian Harm Reduction Network. Freeman AJ, Dore GJ, Law MG, Thorpe M, Von Overbeck J, Lloyd AR, et al. (2001).Estimating progression to cirrhosis in chronic hepatitis C virus infection. Hepatology;34(4Pt 1):809-16.
Greenwood D. ;Slack R. ; Peutherer J ;Barer M (2007). Medical microbiology.seventeenth Ed. Churchili Livingstone Elsevier 738 p.
Harvey, Richard A.; Champe, Pamela C.; Fisher, Bruce D.(2007). Lippincott's Illustrated Reviews Microbiology, 2nd Edition . Lippincott Williams & Wilkins.
•Hutchinson SJ, Bird SM, Goldberg DJ.( 2005).Modeling the current and future disease burden of hepatitis C among injecting drug users in Scotland. Hepatology;42(3):711-23.
Jack, C. Runner, MBA, MT(ASCP), SM(AAM) (2007). Bacterial and viral contamination of reusable sharps containers in a community hospital setting. Vol. 35 No. 8 .527-530.
•Kane,A.;Lloyd,J;Zaffran,M.;Simonsen,L. and Kane,M.(1999).Transmission of hepatitis B,hepatitis C and human immunodeficiency virus through unsafe injection in the developing world:model –based regional estimates .Bulletin of the would health organization .77(10):801-807.
Klatsky AL, Armstrong MA.( 1992).Alcohol, smoking, coffee, and cirrhosis. Am J Epidemiol.;136:1248–1257.
•TenCate V. , Sainz Jr B., Cotler S. , Uprichard S. (2010).Potential treatment options and future research to increase hepatitis C virus treatment response rate.Dovepress J. Hepatic Medicine: Evidence and Research ; 2 : 125–145.
• Wang CS, Chang TT, Yao WJ, and Chou P (2002).Comparison of hepatitis B virus and hepatitis C virus prevalence and risk factors in a community-based study . Am. J. Trop. Med. Hyg., 66(4), pp. 389-393
www.healthinfotranslations.com, (2007). Viral Hepatitis Developed through a partnership of Ohio State University Medical Center, Mount Carmel Health and OhioHealth, Columbus, Ohio.