Role of Interleuken-6 & Tumor necrosis factor – α in Rheumatoid arthritis Patients in Nassiriyah governorate
Abstract
SUMMARY The present study has been done in Al-Nassiriyha, southern Iraq, from December 2006 to August 2007,designed to determine the role of Interleuken-6 and Tumor necrosis factor – α in a group of patients with rheumatoid arthritis for specific period of time and healthy controls, and to show the diagnostic value of IL-6 and TNF-α in relation to rheumatoid factor (RF) to discriminate between those patients with and without RA . Blood samples were taken from 121 patients with rheumatoid arthritis from AL-Hussein Teaching Hospital in AL-Nassiriyah and 120 healthy controls from medical personnel and school students who were healthy . All the information were taken from the patients and controls. An Enzyme-linked immunosorbent assay was used for estimation IL-6, TNF-α and slide agglutination test for the estimation of RF. The seropositivity to IL-6 and TNF-α and RF in patients with rheumatoid arthritis was ( 60% , 56.1% and 57% respectively ) .The study revealed that specificity of TNF- alpha was higher than IL-6 and RF ( 96.6 % , 92.5 % and 81.6 respectively ) .References
Al-Dalaan A, Al Ballaa;S Bahabri;S Biyari T.The Prevalence of Rheumatoid Arthritis in the Qassim Region of Saudi Arabia. Ann Saudi Med 1998; 18 (5):396-397.
St. Clair, E. William; Pisetsky, David S.; Haynes, Barton F: Genetic Determinants.In: Rheumatoid Arthritis, 1st edition .Edited by LippincottWilliams & Wilkins; 2004: Part II-Chapter (10) Pages: 107 – 117.
ACR subcommittee on RA Guidelines: Guidelines for the management of rheumatoid arthritis. Arthritis Rheum 2002, 46:328-46
Steiner G, Smolen J: Autoantibodies in rheumatoid arthritis and their clinical significance.Journal of Arthritis Research 2002, 4(suppl 2):S1-S5
Lene S. Knudsen1, Ib J. Christensen, Tine Lottenburger, Mads N.Pre-analytical and biological variability in circulating interleukin 6 in healthy subjects and patients with rheumatoid arthritis .Journal of Biomarkers, February 2004; 13(1): 59―78
Arend WP, Dayer JM.Cytokines and cytokine inhibitors or antagonists in rheumatoid arthritis. Journal of Arthritis and Rheumatism 1990; 33: 305-315.
Houssiau F, Devogelaer JP, Van DJ, de Deuxchaisnes CN, Van SJ.Interleukin-6 in synovial fluid and serum of patients with rheumatoid Arthritis and other inflammatory arthritides. Journal of Arthritis and Rheumatism 1988;31:784―788.
McInnes IB , Liew FY .2005.Cytokine networks _towards new therapies for Rheumatoid arthritis .Journal of Nature Clinical Practice Rheumatology 2005;1: 31 – 39
Zvezdanovi L, Vidosava , Vladan , Tatjana Cvetkovi . The significance of cytokine in diagnosis of autoimmune diseases . Jugoslov Medical Biochemistry 2006 ; 25 (4) :363 – 372 .
Arnett FC, Edworthy SM, Bloch DA, McShane DJ .The AmericanRheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Journal of Arthritis & Rheumatism1988,31:315-24.
Symmons DPM, Barrett EM, Bankhead CR . The incidence of Rheumatoidarthritis in the United Kingdom: results from the Norfolk Arthritis Register.British Journal of Rheumatology 1994;33:735- 739
Jawed Altaf Baig .Role of Methotrexate in Rheumatoid Arthritis .PhDThesis submitted to University of Karachi , Faculty of Science ,Department of Biochemistry, Karachi.2003 . Available at: www.cpsp.edu.pk/journals/ARCHIEVE/JCPSP-2007/aug07/article10.pdfAccessed in ; 10 /9/2007
Atzeni F. Antibody profiles in patients treated with tumor necrosis factor-alpha antagonists :New findings. PhD thesis submitted to University of Milan,Italy.2006 .Available at:http://www.tdx.cesca.es/TESIS_UAB/AVAILABLE/TDX-0721106
-113709//fa1de1.pdf Accessed in : 22/ 9 /2007
Berglin E .Predictors of Disease Onset and Progression in Early Rheumatoidarthritis ,A Clinical, Laboratory and Radiological Study. Thesis submitted to Department of Public Health and Clinical Medicine, Rheumatology,Umeå University, Sweden. 2006.Available at:
http://www.diva-portal.org/umu/theses/abstract.xsql?dbid=669&lang=sv accessed in: 18 / 5 /2007.
Michael M.Frank , K. Frank Austen , Henry N.Claman , and Emil R.Unanue: Rheumatoid arthritis .In Samter's Immunologic Diseases, Fifth edition .Edited by Mary Babcock ,Nancy Schatken. New York : Volume II ;1995 :Chapter 38 : 699 – 703 .
Koivula, Marja-Kaisa, Autoantibodies binding citrullinated type I and II Collagens in rheumatoid arthritis. Thesis submitted to Faculty of Medicine,Department of Clinical Chemistry, University of Oulu, Finland.2006.of Oulu, Finland Acta Univ. Oul. D 881, 2006 Oulu,Finland. Available at : http://herkules.oulu.fi/isbn9514281101/ accessed in 25/7/2007.
Frédéric A. Houssiau, Jean-Pierre Devogelaer,Jo Van Damme. Interleukin-6
in synovial fluid and serum of patients with rheumatoid arthritis and other
inflammatory arthritides. Journal of Arthritis & Rheumatism 2005 ; 784 – 788 .
Dasgupta B, Corkill M, Kirkham B, Gibson T, Panayi G. Serial Estimation of interleukin 6 as a measure of systemic disease in rheumatoid arthritis. Journal of Rheumatology 1992; 19:22―25.
Houssiau F, Devogelaer JP , Van DJ , de Deuxchaisnes CN , Van SJ .Interleukin- 6 in synovial fluid and serum of patients with rheumatoid Arthritis and other inflammatory arthritides .Journal of Arthritis and Rheumatism 1988; 31:784 – 788 .
Ulfgren AK,Grondal L,Lindblad S,Khademi M,Johnell O,Klareskog L.and Andersson U. lnterindividual and intra-articular variation of proinflammatory cytokines in patients with Rheumatoid arthritis: potential implications for treatment .Journal Annals of the Rheumatic Diseases. 2000 ;59: 439 – 447.
