Inhibitory effect of some plant extracts on growth of some bacteria and fungi pathogens
Abstract
The antimicrobial activity of ethanolic extracts of Pomegranates (Punica granatium L.) pricarps and Celery (Apium graveolens L.), Sweet Clover ( Melilotus officinalis L.),Alfalfa ( Medicago sativa L.), Fenugreek Seed (Trigonella feonum-graecum L.) leaves and Carrot ( Daucus carota L.) seeds against some pathogenic fungi , Gram-negative and Gram-positive bacteria were studied. Also, photochemical compound of extracts were determined, results of the chemical tests explain the M. sativ and P. granatium extracts contain flavonoids, tannins, alkaloids, saponins and glycosides compounds, while the other plants were varied in the kinds of these compounds. Ethanolic extracts show significant differences between most plants extracts, possibly due to deferent in the antimicrobial substance contain. All bacteria which were tested have shown susceptible to the P. granatium and D. carota ethanolic extracts. Proteus vulgaris , Klebsiella aerogenes and Streptococcus aureus the most susceptible bacteria to the D. carota and P. granatium extracts successively, whereas A. graveolens and T. feonum-graecum did not have antibacterial activity against most bacteria. The antifungal activity of the ethanolic extracts of deferent plants show significant differences against pathogens test fungi, D. carota seed and P. granatium pericarps extracts report the most inhibited percentage than with the M.officinalis extract, also, a different level in susceptible of Geotrichum candidum and Trichophton montagrophytes fungi have shown with all extracts which had use.References
Rai,M.K. and Acharya ,D.(1999).Screening of some Asteraceous plant for antimycotic activity. J. Experim. Biol., 15.208-219.
Barnard, M.; Paghitt, M.and Uri,N.D.(1997).Pesticide use and its measuremen. ternatio. Pest Control., 39:161-164.
Nascimento,G.F.;Locatelli,J.;Freita,P.C.andSilva,G.L.(2000).Antimicrobial activity of plant extracts and phytochemical antibiotic resistant bacteria. Braz.J.Microboil. ,31:247-256.
Phongpaichit,S.;Pujenjop,N.;Rukachaisirikul,V.and Ongsakul,M.(2004). Antifungal activity from leaf extracts of Cassia alata L. ,Cassia fistula L. and Cassia tora L. Songklanakarin J.Sci.Tecnol., 26:5. 741-748.
Betoni,J.E.; Mantovani,R.P. ; Barbosa,L.N. ;Di Stasi, L.C.and Junior ,A.F. (2006).Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases .J.Mem.Inst . Oswaldo Crus,Rio de Janeiro.,101:4.387-390.
Karou,D.;Savadago,A.;Canini,A.;Ymeogo,S.;Montesano,C.;Simpori,J.; Collizzi,V. and Traore,A.S.(2006).Antibacterial activity of alkaloids from Side acuta .African J.Biotechnol., 5:2.195-200.
Scalbert,A. (1991).Antimicrobial properties of tannins .J.Phytochem.,30: 3875- 3883.
Letessier ,M.P.;Svoboda ,K.P. and Walter ,D.R. (2001) . Antifungal activity of the essential oil of Hyssop (Hyssopus officinalis ) .J.Pathol., 149:11-12. 673-678.
Prashanth,D.; Asha,M.K. and Amit,A.(2001).Antimicrobial activity of Punica granatum. J.Fitoterapia., 72: 171-173.
Machado,T.B.;Leal,I.C.;Ameral,A.C.;Santos,K.R.N.;Silva,M.G.and Kuster, R.M. (2002).Antimicrobial ellagi tannin of Punica granatium L. fruits . J.Braz.Chem. Soc.,13:606-610.
Fridmen,M.;Henika,P.R.and Mandrell,R.E.(2002).Bactericidal activities of plant essential oils and some of their isolated constituents against Campytobacter jejuni,Esherichia coli,Listeria monocytogenes and Salmonella enterica. J.Food Prot., 65:1545-1560.
Luize,P.S.;Tiuman,T.S.;Morello,L.G.;Maza,P.K.;Uedo-Nakamura,T.;Felho,B.P.D.; Cortez,D.A.G.;Mello,J.C.P.and Nakamura,C.V. (2005). Effect of medicinal plant extracts on growth of Leishmania amazonensis (L.) and Trypanosomacruzi Bara. J.Pharmacutical Sci.41:1.84-94.
irirak,K.;Supawita,T.;Panthong,K.;Voravuthikunchai,S.;Songklanakarin,J.(2005). Antibacterial activity of crude extract of Punica granatum pericarp on pathogenic Gram-Negative bacilli., J.Sci.Technol.,27:2.535 -544.
Voravuthikunchai,S.P.,Sirirak,T.;Limsuwan,S.;Supawita,T.;Iida,T. and Honda,T. (2005).Inhibitory effect of active compounds from Punica granatum pericarp on Verocytotoxin production by Entero- hemorrhagic Esherichia coli 0157:H7. J.Health Sci.,51:5.590-596.
Saxena, J.and Methela,C.S.(1996). Antifungal activity of new compound from Nepata leucophylla and Nepata clarkii. J. Appl.and Environ. Microbiol. 62:2.702-704.
Cruickshank,R.; Duguide,G.P.; Marmion, B.P. and Sawin,R.H.(1975). Medicinal Microbiology .Vol.2:12th. Ed., Living Stone. Edinburgh.UK.
Trease,G.E. and Evans,W.C.(1996).A text book of pharmacognosy.14th ed. Bailliere Tindall Ltd.London.
Harborne,J.B.(1984).Pytochemical methods :Aguide to modern techniques of plant analysis.2nd ed.Chapman and Hall,London .UK.288p.
Al-Khazaragi, S.M. (1991) .Biopharmacological study of Artemisia herba -alba .MSC.Thesis .Univ .Baghdad .
Oloyede,O.O.(2005).Chemical profile of unripe pulp of Carica papaya. Pak. J.Nutri. 4:6.379-381.
Poyrazogula,E.;Gokmen,V.;and Artic,n.(2002).Organic acids and phenolic compound in pomegranates (Punica granatum L.) grown in Turkey .J. Food Composis Ana.,15.567-575.
Schmidt, A. and Mordhorst,T.(2003).Conjugated,cross-conjugated,and psudo-cross-conjugated derivatives of a pyridinium alkaloid from Punica granatum.J.Arkivoc.,(xiv):233-245.
Makoi , J.H.; Nadakidemi ,P.A. (2007) . Biological, ecological and ogronoic significance of plant phenolic compound in rhizophere of the symbiotic legumes . Afri.J. Biotechnol. , 6:12. 1358-1368.
Al- Rawi , A. and Chakravarty ,P. H.L. (1988). Medicinal Plant of Iraq. 2nd. ed. Ministry of Agriculture, Baghdad.
Masaoka , Y.; Kojima , M. ; Sugihara ,S.; Yoshihara , T.; Koshino ,M. and Ichihara , A. (1993) .Dissolution of ferric phosphate by alfalfa (Medicago sativa L. ) root exudates .J. Plant Soil .,155-156 : 175-178.
Phillips, D.A. ; Wery , J. ;Joseph ,C.; Jones , A.D. and Teuber , L.R.(1995). Release of flavonoids and betaines from seeds of seven Medicago species. J.Crop Sci., 35: 805-808 .
Zin , L.Z. ;He ,X.G. ;Lindenmaier , M.; Yong , T.; Cleary ,M.;Qiu ,S.X. and Cordell , G.A. ( 2000) . LC-EST-MS study of flavonoid glycoside malonates of red Clover ( Trifolium paratense ) .J. Food Chem., 48: 354-365.
Heisey,R.M.and Gorham,B.K.(1992).Antimicrobial effects of plant extracts of Streptococcus mutans ,Candidia albicans,Trichophton rubrum and other micro -organisms . J.Letters in Appl. microbiol.,14:136-139.
Anesini, E.and Perez, C.(1993).Screening of plants used in Argentine folk medicine for antimicrobial activity .J.Ethnopharmocol.,39:119-128.
Tassou, C.C.; Koutsoumanis, K.and Nychas,G.J.(2000).Inhibition of Salmonella enteridis and Staphaloccus aureus on nutrient broth by mint essential oil. J.Food Res.Internat. 48:273-280.
Jasica-Misiak ,I.; Lipok , J.; Nowakowska ,E.M. ;Wieczorek, P.P.;Mlynarz,P. and Kafarski , P. (2004) . Antifungal activity of the Carrot seeds oil and its major sesquiterpene compounds. J.Naturfosch., 59c:791-796.
Anitha , R. and Kannan , P. ( 2005) .Antifungal activity of Clerodendrum Inerme (L.) and Clerodendrum phlomidis (L.).,Turk. J. Biol.,30:139-142.
Hernandez .I.; Portieles , R. ; Chacon , O. and Hidalgo, O.B. (2005). Proteins and peptides for the control of phytopathogenic fungi. J. Biotecnol. Aplica. ,22 : 256-260 .
Kerem, Z.; Shashoua, H. and Yarden ,O. (2005) .Microwave- assisted extraction of bioactive saponins from Chick pea ( Cicer arietinum L.). J. Sci. Food Agric., 85: 405-412.
Raddic J.G.;Prysdale,R.B.(1984).Destabilization of liposome membrane by the - steroidal glycoalkaloid a-tomotine.J.Phytochem.23:9-25.
Osbourn , A. E. (2003) .Saponin in Cereals . , J.Phytochem., 62: 1-4.
Guittat ,L.; Alberti ,P.; Rosu ,F. ;Van Miert ,S. ;Thetiot ,E.;Pieters,L.; Gabelica,V.; De Pauw ,E. ;Ottaviani ,A.; Roiu ,J.F.and Mergny ,J.L. (2003) .Interaction of cryptolepine an neocryptolepine with unusual DNA structures .J.Biochem., 85: 535-541.
Nawwar,M.;Sahar,A.;Hussein,M.and Merfort,I.(1994).NMR spectral analysis of polyphenol from Punica granatium .J.Phytochem.,36:793- 798.