Detection of Human Cytomegalovirus Pp65 in colorectal adeno- carcinoma and villous adenoma using Immunohistochemistry (IHC) technique
Keywords:
Thi-Qar, Human Cytomegalovirus, colorectal adenocarcinoma, Immunohistochemistry (IHC)Abstract
Colorectal adenocarcinoma is the third most common cancer in men and women and is the second leading causes cancer death. Recently detected Human Cytomegalovirus (HCMV) proteins in a high proportion of human colorectal tumors. These finding raise the chance of whether persistent HCMV infection can induce oncogenic pathways that eventuate in colorectal adenocarcinoma. This study investigates whether HCMV participates in human colorectal tumorigenesis by the detection of HCMV Pp65 within epithelial cells of colorectal carcinoma using Immunohistochemistry (IHC). We obtained formalin–fixed, paraffin – embedded specimens of adenocarcinoma, villous adenoma, and normal tissues from the margins of the excision as a control. In this study, 55 specimens were classified into three groups: Adenocarcinoma, Villous adenoma, and control group, all groups have been tested by IHC to detect the presence of HCMV proteins using mouse monoclonal antibodies to an early protein (pp65). The results of IHC assay showed specific nuclear and cytoplasmic reaction of HCMV proteins within the epithelial cells of colorectal adenocarcinoma (75.75%), and villous adenoma (83.33%), in addition to that no nuclear or cytoplasmic reaction were showed in any case of control group. In view of the many cellular modulatory properties of this virus, our data justify further studies to establish whether HCMV interfere with the pathogenesis of colorectal adenocarcinoma.References
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