Molecular Analysis of Virulence Genes Lux S, Rsb A and Mrpa in Proteus Mirabilis Isolated from Different Clinical Samples
Keywords:
Proteus mirabilis, lux s, rsm A and mrp A genes detection, Vitek 2 systemAbstract
: The research endeavour involved the collection of a total of 100 samples from diverseclinical origins. Specifically, 70 urine samples were obtained from patients, while 20 samples were
derived from wounds. Additionally, 10 samples were acquired through ear swabs from patients enrolled
in private clinics, Imam Sadiq Hospital, and Al-Hilla Hospitals. The data collection period spanned from
November 2023 to December 2024.
Out of the hundred samples that were analyzed, it was observed that a significant majority, specifically
eighty samples (80%), exhibited positive bacterial cultures. Conversely, a smaller proportion of the
samples, precisely twenty samples (20%), did not exhibit any growth of bacteria. The findings of this
study indicate the potential existence of microorganisms, including viruses, fungi, and other anaerobic
agents, which could pose challenges in terms of culturing or may be attributed to variations in the sizes
and compositions of the samples. Utilizing the Vitek 2 methodology, selective media, and biochemical
assays, it was observed that out of the eighty-nine samples analyzed, a subset of twenty samples
exhibited positive outcomes, suggesting Proteus mirabilis presence. The detection of luxS, rsmA, and
mrpA genes in Proteus mirabilis was accomplished using the polymerase chain reaction (PCR)
technique.
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