Molecular Detection of Some Virulence Genes among Staphylococcus aureus Isolated from Skin Infections
Keywords:
Acne, impetigo Staphylococcus aureusAbstract
The study included the collection of (100) samples were collected from differentskin sites(Acne, impetigo, cellulitis, folliculitis). Clinical samples were collected from patients
who were admitted and visit in Al- Imam Al-Sadiq Hospital and Maternity and Children's
Hospital in Al-Hilla city, at the period from October (2023) to January (2024). Twenty isolates
were showed positive and identified as Staphylococcus aureus by using selective media,
biochemical tests and Vitek 2 system. and it was found that, out of the total 100 samples,
89(89%) samples showed positive bacterial culture. No growth was seen in other 11(11%)
samples, which indicate the presence of microorganisms that may be cultured with difficulty
such as virus, fungi and other anaerobic agents or may be due to difference in the size and nature
of the samples. Among (89) positive culturing selective media, 89/100 positive cultures ,were
divided into 48/89 (53.93%) Gram-negative bacteria, and 41/89 (46.07%) Gram-positive bacteria
based on Gram stain and culture medium. Among Gram positive bacteria 20/41 (48.78%),
isolates of Staphylococcus aureus were obtained. icaA, and fnbA were detected by PCR
amplification, fnbA was found in 70% of the isolates while icaA 60%. and 191 bp 689 were
considered positive for the presence of fnbA ,and icaA respectively.
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