Isolation and Identification of Gram Positive and Gram Negative Bacteria Causing Dental Caries in Children in Thi Qar province

Authors

  • Nawal Fahaed Diash Department of Microbiology, Medical Microbiology, College of Medicine, University of Thi-Qar
  • Faten Naeem Abbas Department of Microbiology, Medical Microbiology, College of Medicine, University of Thi-Qar

Keywords:

Dental Caries, cariogenic bacteria

Abstract

Background: Dental caries is an infectious disease-causing tooth demineralization and cavities. Dental caries in
children is caused by the combination of three major factors carbohydrates, the presence of caries bacteria on dental
plaque, and the rapid impact of hard tooth surfaces. The mouth contains a large number of bacteria, including a few
species that cause dental caries called cariogenic bacteria. Gram positive bacteria are the main contributors to caries in
the teeth
Methods: A study was conducted in Thi-Qar province between August and December 2023 AD, involving 100
swabs and dental plaque samples from (70 patients and 30 controls) aged 3-11 years. The samples were processed and
cultivated in a specific culture medium.
Conclusions: The current investigation demonstrated a high rate of Gram-positive bacteria isolated from dental
cavities in children aged 3 to 11 years. Gram stain, biochemical tests, and VITEK 2 compact results showed that grampositive bacteria accounted for 81% (180 isolates) and gram-negative bacteria for 19% (42 isolates). There were 109
isolates with typical Streptococcus colony morphologies

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Published

2024-05-12

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