The Antibacterial Activity of Silver Nanoparticles and Detection of the Fnba Gene in S. Aureus Isolated from Burn Patients

Authors

  • Reyam Majeed Hassan Department of Microbiology, College of Medicine, University of Thi-Qar, 64001, Iraq
  • Zainab Dakhil Degaim Department of Microbiology, College of Medicine, University of Thi-Qar, 64001, Iraq

Keywords:

S. aureus, burn, Ag NPs, MIC, fnbA gene

Abstract

: The purpose of the recent study was to profile of biofilm formation gene (fnbA) in S.
aureus which isolated from burn patients, and to detect the antibacterial activity and MICs of
silver nanoparticles against pathogenic S. aureus isolated from burn wounds. One hundred and
sixty samples from burn patients were collected from August to October 2023 in a burn center in
the Thi-Qar province of Iraq. The S. aureus was only identified in (35; 21.90%) isolates.
Microscopical examination, morphological characterization, diverse biochemical testes, were
used to recognize S. aureus isolates. The PCR technique results recorded that 42.8% of S. aureus
isolates had the fnbA gene. The Ag NPs had antibacterial activity against completely S. aureus
isolates.

References

-Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clinical

microbiology reviews. 2006 Apr;19(2):403-34.

- Kwiecinski JM, Horswill AR. Staphylococcus aureus bloodstream infections: pathogenesis and

regulatory mechanisms. Current opinion in microbiology. 2020 Feb 1; 53:51-60.

- Gjødsbøl K, Christensen JJ, Karlsmark T, Jørgensen B, Klein BM, Krogfelt KA. Multiple

bacterial species reside in chronic wounds: a longitudinal study. International wound journal.

Sep;3(3):225-31.

- Fazli M, Bjarnsholt T, Kirketerp-Møller K, Jørgensen B, Andersen AS, Krogfelt KA, Givskov

M, Tolker-Nielsen T. Nonrandom distribution of Pseudomonas aeruginosa and Staphylococcus

aureus in chronic wounds. Journal of clinical microbiology. 2009 Dec;47(12):4084-9.

- Pourhajibagher M, Mahmoudi H, Rezaei-Soufi L, Alikhani MY, Bahador A. Potentiation

effects of antimicrobial photodynamic therapy on quorum sensing genes expression: A promising

treatment for multi-species bacterial biofilms in burn wound infections. Photodiagnosis and

photodynamic therapy. 2020 Jun 1; 30:101717.

- Goodwine J, Gil J, Doiron A, Valdes J, Solis M, Higa A, Davis S, Sauer K. Pyruvate-depleting

conditions induce biofilm dispersion and enhance the efficacy of antibiotics in killing biofilms in

vitr o and in vivo. Scientific reports. 2019 Mar 6;9(1):3763.

- Speziale P, Pietrocola G. The multivalent role of fibronectin-binding proteins A and B (FnBPA

and FnBPB) of Staphylococcus aureus in host infections. Frontiers in microbiology. 2020 Aug

; 11:2054.

- Speziale P, Pietrocola G. The multivalent role of fibronectin-binding proteins A and B (FnBPA

and FnBPB) of Staphylococcus aureus in host infections. Frontiers in microbiology. 2020 Aug

; 11:2054.

- Saravanan M, Barik SK, MubarakAli D, Prakash P, Pugazhendhi A. Synthesis of silver

nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against

pathogenic bacteria. Microbial pathogenesis. 2018 Mar 1; 116:221-6.

- Oves M, Aslam M, Rauf MA, Qayyum S, Qari HA, Khan MS, Alam MZ, Tabrez S,

Pugazhendhi A, Ismail IM. Antimicrobial and anticancer activities of silver nanoparticles

synthesized from the root hair extract of Phoenix dactylifera. Materials Science and Engineering:

C. 2018 Aug 1; 89:429-43.

-Samuel MS, Jose S, Selvarajan E, Mathimani T, Pugazhendhi A. Biosynthesized silver

nanoparticles using Bacillus amyloliquefaciens; Application for cytotoxicity effect on A549 cell

line and photocatalytic degradation of p-nitrophenol. Journal of Photochemistry and

Photobiology B: Biology. 2020 Jan 1; 202:111642.

- Avalos A, Haza AI, Mateo D, Morales P. Interactions of manufactured silver nanoparticles of

different sizes with normal human dermal fibroblasts. International wound journal. 2016

Feb;13(1):101-9.

- Blecher K, Nasir A, Friedman A. The growing role of nanotechnology in combating

infectious disease. Virulence. 2011 Sep 1;2(5):395-401.

- Landini P, Antoniani D, Burgess JG, Nijland R. Molecular mechanisms of compounds

affecting bacterial biofilm formation and dispersal. Applied microbiology and biotechnology.

Apr;86:813-23.

- Kwakye‐Awuah B, Williams C, Kenward MA, Radecka I. Antimicrobial action and

efficiency of silver‐loaded zeolite X. Journal of Applied Microbiology. 2008 May

;104(5):151624.

- Singh JP, Singh V, Sharma A, Pandey G, Chae KH, Lee S. Approaches to synthesize MgO

nanostructures for diverse applications. Heliyon. 2020 Sep 1;6(9).

- Qu X, Wang S, Qu Y, Wang H, Ye X, Tang L, Xie Q. Antimicrobial Susceptibility

Characteristics and Risk Factors Associated with Adult Sepsis in Wenzhou, China. Infection and

Drug Resistance. 2022 Jan 1:915-24.

- Abraham NM, Jefferson KK. A low molecular weight component of serum inhibits biofilm

formation in Staphylococcus aureus. Microbial pathogenesis. 2010 Dec 1;49(6):388-91.

- Bessa LJ, Fazii P, Di Giulio M, Cellini L. Bacterial isolates from infected wounds and their

antibiotic susceptibility pattern: some remarks about wound infection. International wound

journal. 2015 Feb;12(1):47-52.

- Kot B, Sytykiewicz H, Sprawka I. Expression of the biofilm-associated genes in methicillinresistant Staphylococcus aureus in biofilm and planktonic conditions. International journal of

molecular sciences. 2018 Nov 6;19(11):3487

- Kazemzadeh J, Rabiepoor S, Alizadeh S. The quality of life in women with burns in Iran.

World journal of plastic surgery. 2019 Jan;8(1):33.

- Rahim Hateet R. Isolation and Identification of Some Bacteria Contemn in Burn Wounds in

Misan, Iraq. Archives of Razi Institute. 2021 Dec 1;76(6):1665-70.

- Tsurumi A, Que YA, Ryan CM, Tompkins RG, Rahme LG. TNF-α/IL-10 ratio correlates

with burn severity and may serve as a risk predictor of increased susceptibility to infections.

Frontiers in public health. 2016 Oct 5; 4:216.

- Degaim ZD, Shani WS, Hamim SS. Virulence factors of Methicillin Resistant

Staphylococcus aureus (MRSA) isolated from burn patients. International Journal of Current

Microbiology and Applied Sciences. 2015;4(7):898-906.

susceptibility pattern in Tabriz, Iran. Arch Pharm Pract. 2020;11(S1):137-43

- VázquezSánchez, D., Cabo, M.L., Ibusquiza, P.S., and RodríguezHerrera, J.J.,

Biofilmforming ability and resistance to industrial disinfectants of Staphylococcus aureus

isolated from fishery products, Food Control, 2014, vol. 39, pp. 8–16

- Violante TL, Haase EM, Vickerman MM. Collagen-binding streptococcal surface proteins

influence the susceptibility of biofilm cells to endodontic antimicrobial solutions. Journal of

Endodontics. 2013 Mar 1;39(3):370-4.

- Goldmann O, Tuchscherr L, Rohde M, Medina E. α‐hemolysin enhances Staphylococcus

aureus internalization and survival within mast cells by modulating the expression of β1 integrin.

Cellular microbiology. 2016 Jun;18(6):807-19.

- Mohammadi A, Goudarzi M, Dadashi M, Soltani M, Goudarzi H, Hajikhani B. Molecular

detection of genes involved in biofilm formation in Staphylococcus aureus strains isolates:

evidence from shahid motahari hospital in Tehran. Jundishapur Journal of Microbiology. 2020 Jul

;13(7).

- Mirzaee M, Najar-Peerayeh S, Behmanesh M. Prevalence of fibronectin-binding protein

(FnbA and FnbB) genes among clinical isolates of methicillin resistant Staphylococcus aureus.

Molecular genetics, microbiology and virology. 2015 Oct; 30:221-4.

- Chen X, Wu Z, Zhou Y, Zhu J, Li K, Shao H, Wei L. Molecular and virulence characteristics

of methicillin-resistant Staphylococcus aureus in burn patients. Frontiers in Laboratory Medicine.

Mar 1;1(1):43-7.

- Eftekhar F, Rezaee R, Azad M, Azimi H, Goudarzi H, Goudarzi M. Distribution of adhesion

and toxin genes in staphylococcus aureus strains recovered from hospitalized patients admitted to

the ICU. Archives of Pediatric Infectious Diseases. 2017 Jan 1;5(1).

- Abad HE, Sadeghi J, Aghazadeh M, Ahangarzadeh Rezaee M, Samadi Kafil H, Ahangar

Oskouee M. Frequency of fnbA, fnbB, hla and cna genes in Staphylococcus aureus isolates

obtained from blood cultures and their antimicrobial susceptibility pattern in Tabriz, Iran. Arch

Pharm Pract. 2020;11(S1):137-43

- Mater AD, Degaim ZD, Abdulazeez AS. Detection of Fibronectin Binding Protein (fnb A)

gene in Staphylococcus aureus isolates. University of Thi-Qar Journal. 2019 Apr 25;14(1):10-6.

- Morone MV, Dell'Annunziata F, Giugliano R, Chianese A, De Filippis A, Rinaldi L,

Gambardella U, Franci G, Galdiero M, Morone A. Pulsed laser ablation of magnetic

nanoparticles as a novel antibacterial strategy against gram positive bacteria. Applied Surface

Science Advances. 2022 Feb 1;7:100213.

- Al-Dhabi NA, Mohammed Ghilan AK, Arasu MV. Characterization of silver nanomaterials

derived from marine Streptomyces sp. al-dhabi-87 and its in vitro application against multidrug

resistant and extended-spectrum beta-lactamase clinical pathogens. Nanomaterials. 2018 Apr

;8(5):279.

- Parvekar P, Palaskar J, Metgud S, Maria R, Dutta S. The minimum inhibitory concentration

(MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against

Staphylococcus aureus. Biomaterial investigations in dentistry. 2020 Jan 1;7(1):105-9.

- Viorica RP, Pawel P, Kinga M, Michal Z, Katarzyna R, Boguslaw B. Lactococcus lactis as a

safe and inexpensive source of bioactive silver composites. Applied Microbiology and

Biotechnology. 2017 Oct;101:7141-53

Downloads

Published

2024-05-14

Issue

Section

Articles