Alogliptin Alleviates Ischemia/ Reperfusion Induced Kidney Injury by Attenuating Inflammation and Apoptosis in Rats Model
Keywords:
Alogliptin, ischemia/reperfusion, urea, creatinine, Kim-1Abstract
Ischemia/reperfusion injury is a complicated phenomenon leading to cellular damage via thebiphasic process. Ischemia causes injury by reducing or entirely eliminating the energy supply required
for homeostasis. Reperfusion exacerbates cellular damage through several proposed mechanisms,
including inflammatory responses and the production of free radicals and oxygen. A total of 24 adult male
rats were randomly divided into four groups(n=6). Sham group: Rats were anesthetized and had a
surgery without ischemia-reperfusion induction. I/R control group: Rats underwent a same anesthetic
procedure, followed by 40 min bilateral renal ischemia, then 2 hr. of reperfusion. DMSO group: Rats
were received DMSO (as a solvent for alogliptin) ip injection at 24hr and 30 min. before surgery (an
identical surgical technique as the control group). Alogliptin treated group: Rats were received
alogliptin ip injection 10 mg/kg at 24h and 30 min. before surgery (an identical surgical technique as the
control group). All rats had their blood samples and renal tissues collected the conclusion of the
reperfusion phase. In blood samples, serum urea and creatinine were measured by the standard laboratory
protocols, and by using ELISA method, KIM-1 measurement was taken in kidney tissues. Furthermore,
kidney histopathological investigation was conducted by assessing the extent of degeneration, tubular
dilation, interstitial lymphocyte infiltration, and loss of brush borders. It was concluded that kidney
damage resulted from elevated urea, creatinine, and KIM-1 levels noted in blood samples and kidney
tissues of rats in both control and DMSO groups in contrast with sham group. In comparison to the
control and DMSO groups, rats in the treatment group showed decreasing levels of urea, creatinine, and
KIM-1, Furthermore, the treated group with alogliptin also had decreased histopathological damage
scores. these results suggest that alogliptin can prevent kidney damage as mainly evidenced by the
decreased histopathological damage region, enhanced renal functions. Alogliptin's characteristics may
facilitate its application in the management of acute kidney injury (AKI).
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