In Vitro Antimicrobial Activity and GC-MS Analysis of Crude Aqueous Methanolic Extract Produced from Leaves of Eucalyptus species

Authors

  • Dhurgham A .H. Alhasan College of Veterinary Medicine, University of Thi-Qar, Iraq.
  • Husein A. Husein Pharmacy College, University of Thi-Qar, Iraq.
  • Majid A. Z. Albadry 3Education Directorate of Thi-Qar,Ministry of Education, Iraq

Keywords:

Leaves of Eucalyptus sp, Antimicrobial Activity, GC-MS Analysis

Abstract

Objectives:To evaluate the antimicrobial activity of the aqueous methanolic extract was given by the leaves of Eucalyptus sp.growing in Thi-Qar Province, south of Iraq. Additionally, to detect the compounds within the extract by using the technique of gas chromatography-mass spectrometry (GC-MS). Methodology: The healthy leaves of Eucalyptus sp. were collected, washed with tap water then by distilled water, dried, and pulverized through using a small mill to make the dried leaves as a powder. The leaves powder was extracted by absolute petroleum ether then aqueous methanol (80%) for obtaining a sticky crude extract of the aqueous methanol filtrate. The crude extract was tested against some microbial pathogens as well as analysis of the extract using the technique of GC-MS and phytochemical tests. Results:The current study showed the antimicrobial activity of the crude aqueous methanolic extract obtained from Leaves ofEucalyptus sp. by which 40000 µg∕ml of the extract exhibited inhibition zones around Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Klebsiella sp. besides Candida albicans that the inhibition zones were measured to be 30, 27, 26, 30, and 38 mm respectively. The chemical tests appeared the crude extract containsalkaloids, glycosides, flavonoids, tannins, andsaponin glycosides. GC-MS analysis detected constituents of the extract are dimethylsulfoxoniumformylmethylide, diethyl phthalate, benzene,1,1'-(1,2-cyclobutanediyl)bis-trans-,diisoctyladipate, and 2-methyl-7-phenylindole. Conclusion: The present study concluded that the leaves ofEucalyptussp. possess the bioactive products which are needed to be separatedas the pure compoundsby using techniques of chromatography in order to separately tested in vitro and in vivo, then application of spectrometry techniques for characterization of their chemical structure. Finally, these compounds can be used to treat diseasesas drugs in hospitals.

References

-Wolfender, J.L.; Eugster, P.J.; Bohni, N.and Cuendet, M. (2011). Advanced Methods for Natural Products Discovery in the Field of Nutraceuticals. Chimia., 6: 400–406.

-Newman, D.J. and Cragg, G.M. (2012).Natural Products as Sources of New Drugs over the 30 Years from 1981 to 2010. J. Nat. Prod., 75: 311–335.

-Dhingra, O.D and Sinclair, J.B. (1995). Basic Plant Pathology Methods. London, CRC Press. P: 287.

-Babayi, H.; Kolo, I.; Okogun, J.I. and Ijah, U.J.J. (2004).The Antimicrobial Activities of Methanolic Extracts of Eucalyptus camaldulensis and Terminalia catappaAgainst Some Pathogenic Microorganisms.Biokem., 16(2):106-111.

-Adeniyi, B. A.; Lawal, T. O. and Olaleye, S.B. (2006). Antimicrobial and Gastroprotective Activities of E.camaldulensis Crude Extracts. J.Biosci., 6(6):1141-1145.

-Pandey, V.B. and Bhattacharya, S.K.(2007). Scientific Appraisal of Rudraksha (E. camaldulensis) Chemical and Pharmacological Studies. J. Res. Edu. in Indian Med., 4: 47-50.

-Sytsma, K.J.; Litt, A.; Zjhra, M.L.; Pires, C.; Nepokroeff, M.; Conti, E.; Walker, J. and Wilson, P.G. (2004).Clades, Clocks, and Continents: Historical and Biogeographical Analysis of Myrtaceae, Vochysiaceae, and Relatives in the Southern Hemisphere. Int. J. Plant Sci., 165: S85–S105.

-Brooker, M.I.H. and Kleinig, D.A. (2006).Field Guide to Eucalypts, Volume 1, South-eastern Australia, 3rd. ed., Bloomings Books Pty Ltd, Richmond, VA, USA.353P.

-Bachir, R.G.and Benali, M. (2012).Antibacterial Activity of The Essential Oils from The Leaves of Eucalyptus globulus Against Escherichia coli and Staphylococcus aureus. Asian Pac. J. Trop. Med., 2: 739–742.

-Singh, H.P.; Kaur, S.; Negi, K.; Kumari, S.; Saini, V.; Batish, D.R.and Kohli, R.K. (2012).Assessment of in vitro Antioxidant Activity of Essential Oil of Eucalyptus citriodora (lemon-scented Eucalypt; Myrtaceae) and Its Major Constituents. LWT-Food Sci. Technol., 48: 237–241.

-Boulekbache-Makhlouf, L.; Slimani, S.and Madani, K. (2013). Total Phenolic Content, Antioxidant and Antibacterial Activities of Fruits of Eucalyptus globulus Cultivated in Algeria. Ind. Crops Prod., 41: 85–89.

-Luis, A.; Neiva, D.; Pereira, H.; Gominho, J.; Domingues, F. and Duarte, A.P. (2014).Stumps of Eucalyptus globulus as a Source of Antioxidant and Antimicrobial Polyphenols. Molecu., 19: 16428–16446.

-Pereira, V.; Dias, C.; Vasconcelos, M.C.; Rosa, E. and Saavedra, M.J.(2014). Antibacterial Activity and Synergistic Effects between Eucalyptus globulus Leaf Residues (Essential Oils and Extracts) and Antibiotics Against Several Isolates of Respiratory Tract Infections (Pseudomonas aeruginosa). Ind. Crops Prod., 52: 1–7.

-Wigmore, S.M; Naiker, M. and Bean, D.C. (2016). Antimicrobial Activity of Extracts from Native Plants of Temperate Australia. Pharmacog. Commn., 6(2): 80-84.

-Clarke, P. (1987).Aboriginal Uses of Plants as Medicines, Narcotics, and Poisons in Southern South Australia. J. Anthropol. Soci. of South. Austr., 25(5):3-22.

-Barr, A.; Chapman, J.; Smith, N. and Beveridge M. (1988).Traditional Bush Medicines and Aboriginal Pharmacopoeia, (Greenhouse Publications Pty Ltd. Australia, Rich-Mond,). Cited by Wigmore, S.M; Naiker, M. and Bean, D.C. (2016). Antimicrobial Activity of Extracts from Native Plants of Temperate Australia. Pharmacog. Commn., 6(2): 80-84.

-von Martius, S.; Hammer, K.A, and Locher, C. (2012). Chemical Characteristics and Antimicrobial Effects of Some Eucalyptus kinos. J. Ethnopharmacol., 144(2):293-9.

-Chang, C.C.; Yang, M.H.; Wen, H. and Chern, M. J. C. (2002). Estimation of Total Flavonoid Content in Propolish by Two Complementary Colorimetric Methods. J. Food Drug Anal., 10 (3): 178-82.

-Shah, B and Seth, A.(2010).Textbook of Pharmacognosy and phytochemistry. 1st ed. Elsevier, Kundli, Haryana, India: Pp:578.

-Sabri, F. Z.; Belarbi, M., Sabri, S. and Alsayadi, M. M.S. (2012). Phytochemical Screening and Identification of Some Compounds from Mallow. J. Nat. Prod. Plant. Resour., 2 (4):512-516.

-Gupta, M.; Thakur,S.; Sharma,A. and Gupta,S. (2013). Qualitative and Quantitative Analysis of Phytochemicals and Pharmacological Value of Some Dye Yielding Medicinal Plants, Orient. J. Chem., 29 (2): 475-481.

-Rahman, M. A.; Rahman M. A. and Ahmed, N.U. (2013). Phytochemical and Biological Activities of Ethanolic Extract of Cassia hirsuta leaves., Bangladesh. J. Sci. Ind. Res., 48(1):43-50.

-Saroya, A.S.(2011). Herbalism, Phytochemistry, and Ethnopharmacology. CRC Press, Taylor & Francis Group, Science Publishers, USA. P:3.

-Kumoro, A. C.; Hasan, M. and Singh,H. (2009).Effects of Solvent Properties on The Soxhlet Extraction of Diterpenoid Lactones from Andrographis paniculata leaves. Sci. Asia., 35:306–309.

-Altemimi, A.; Lakhssassi, N.; Baharlouei, A.; Watson, D.G. and Lightfoot, D.A.(2017).Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants. 6(42): 1-23.

-Ahmad, I.; Mehomood, Z. and Mohammed, F.(1998). Screening of Some Indian Medicinal Plants for Their Antimicrobial Properties. J. Ethnopharma. 62(2): 183 – 193.

-Pamplona–Roger, G. D.(1999). Encyclopedia of Medicinal Plants. Vol. 1 and 2, (2 ed.).Education and Health Library, the European Union, UK. Pp: 128 – 150.

-Shariff, Z. U.(2001). Modern Herbal Therapy for Common Ailments. Nature Pharmacy Series (Volume 1), Spectrum Books Limited, Ibadan, Nigeria in Association with Safari Books (Export) Limited, U K. Pp: 9 – 84.

-Anonmymous. (1985). Final Report on The Safety Assessment of Dibutyl phthalate, Dimethylphthalate, and Diethyl phthalate. J. Ameri. College of Toxicol., 4(3): 267-303.

-Kamrin, M.A. and Mayor, G.H. (1991). Diethyl Phthalate-A Perspective. J. Clin. Pharmacol., 31(5):484–489.

-Wahl, H.G.; Hoffmann, A.; Häring, H.U. and Liebich, H.M. (1999).Identification of Plasticizers in Medical Products by A Combined Direct Thermodesorption-Cooled Injection System and Gas Chromatography-Mass Spectrometry. J.Chromatog., A, 847:1–7.

-World Health Organization,WHO.(2003).Concise International Chemical Assessment Document 52, Diethyl Phthalate. WHO Library Cataloguing-in-Publication Data. P:6.

-Khyade, M. Sh. and Waman, M.B. (2017). Chemical Profile and Antioxidant Properties of Mundulea sericea. Pharmacog. J., 9(2): 213-220.

-Ankita, Sh.; Tribhuwan, S. and Rekha, V.(2015). GC-MS Analysis of Bioactive Phytoconstituent from Rumex vesicarius L. Int. Res. J. Pharma., 6(4):269-272.

-Ajayi, G.O.; Olagunju, J.A.; Ademuyiwa, O. and Martins, O.C. (2011).Gas Chromatography-Mass Spectrometry Analysis and Phytochemical Screening of Ethanolic Root Extract of Plumbago zeylanica, Linn. J. Medic. Plants. Res., 5(9):1756-1761.

-Parthipan, B.;, Suky, M.G.T. and Mohan, V.R. (2015). GC-MS Analysis of Phytocomponents in Pleiospermium alatum (Wall. ex Wight & Arn.) Swingle, (Rutaceae). J. Pharmacog. Phytochem., 4(1): 216-222.

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Published

2019-08-02

Issue

Vol. 17 No. 1 (2019)

Section

ARTICLE