Study Of TiO2 Nanoparticles Induction For Biological System

  • Lames Majid Hammed Thi-Qar University, College of medicine
  • Hassan Tuhmaz Hamad Thi-Qar University, College of medicine
Keywords: TiO2 nanoparticles, Cell viability, HEK-293


Background:   The biological activity of NPs strongly depends on physicochemical parameters but these are not routinely considered in toxicity screening, such as dose metrics which is studied by many researchers.Titanium dioxide nanoparticles (nano-TiO2) are extensively used in the cosmetics, pharmaceutical, and paint industries as a coloring material due to its high stability, anticorrosion and photocatalytic properties Aim:    This study represents the study of TiO2  nanoparticles induction for biological systems.                                                                                                                                        Method:Curve fitting in matlab program is used to study the effect of parameters of the system on the cell levels with existence TNPs.                                                                            Results: The Consequences showed that nanoparticles induce human embryonic kidney cells (HEK-293) the generation of reactive oxygen species (ROS) followed by significant depletion of glutathione levels and increased lipid peroxidation. From the results, we observe that the behavior of can be predicated by estimating the     equations which described the variation of culler effecting by increased the TNPs concentration at different exposure times.            


- Bhattacharya, K.; Davoren, M.; Boertz, J.; Schins, R.P.; Hoffmann, E.; Dopp, E. Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in humanlung cells. Part. FibreToxicol. 2009, 6, 17.

- Geiser M, Rothen-Rutishauser B, Kapp N, Schuerch S, Kreyling W, Schulz H, Semmler M, Im HV, Heyder J, Gehr P. Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells. Environtal Health Perspectives. 2005; 113:1555-60.

- Ramovatar Meena1, Ruchita Pal2 , Surya Narayan Pradhan1, Madhu Rani and R. Paulraj, Comparative study of TiO2 and TiSiO4 nanoparticles induced oxidative stress and apoptosis of HEK-293 cells , Adv. Mat. Lett. 2012, 3(6), 459-465.

- Chithrani BD, Chan WCW. Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of

different sizes and shapes. Nano Letters. 2007; 7:1542-50.

- GurrJR,Wang AS, Chen CH, Jan KY. Ultrafine titanium dioxide particles in the absence of photoactivation can induce

oxidative damage to human bronchial epithelial cells. Toxicology. 2005; 213, 66–73.

- Noël, A.; Maghni, K.; Cloutier, Y.; Dion, C.; Wilkinson, K.J.; Hallé, S.; Tardif, R.; Truchon, G.Effects of inhaled nano-TiO2 aerosols showing two distinct agglomeration states on rat lungs.

Toxicol. Lett. 2012, 214, 109–119.

- Noël, A.; Cloutier, Y.; Wilkinson, K.J.; Dion, C.; Hallé, S.; Maghni, K.; Tardif, R.; Truchon, G.Generatingnano-aerosols from TiO2 (5 nm) nanoparticles showing different agglomeration states.

Application to toxicological studies. J. Occup. Environ. Hyg. 2013, 10, 86–96.

- Noël, A.; Charbonneau, M.; Cloutier, Y.; Tardif, R.; Truchon, G. Rat pulmonary responses to

inhaled nano-TiO2: Effect of primary particle size and agglomeration state. Part. FibreToxicol.2013, 10, 48.

- Wang, J.; Fan, Y.; Gao, Y.; Hu, Q.; Wang, T. TiO2 nanoparticles translocation and potential toxicological effect in rats after intraarticular injection. Biomaterials 2009, 30, 4590–4600.

- Li, B.; Ze, Y.; Sun, Q.; Zhang, T.; Sang, X.; Cui, Y.; Wang, X.; Gui, S.; Tan, D.; Zhu, M.; Molecular mechanisms of nanosized titanium dioxide-induced pulmonary injury in mice.PLoS One 2013, 8, e55563.

- Soto K, Garza KM, Murr LE: Cytotoxic effects of aggregated nanomaterials. Acta Biomaterials. 2007; 3:351-8.

- Park S, Lee YK, Jun, M, Kim KH, Chung N, Ahn EK, Lim Y, Lee KH. Cellular toxicity of various inhalable metal

nanoparticles on human alveolar epithelial cells. Inhalation Toxicology. 2007; 19:59–65.