Oxidative Stress Status In Patients With Rheumatoid Arthritis
Keywords:Rheumatoid arthritis, MDA, catalase, superoxide dismutase, glutathione peroxidase
AbstractBackground: Rheumatoid arthritis (RA) is the most common form of inflammatory arthritis, which is an autoimmune disease characterized by chronic inflammation of synovial joints, ultimately leading to joint destructions and permanent disability. In RA oxidative stress are impaired (which caused by free radicals) might have an essential role in the etiology of RA. Objectives: The objective of this study was to determine oxidative stress by measuring malondialdehyde and enzymatic status by estimating superoxide dismutase, catalase and glutathione peroxidase in patients of RA and then comparing with healthy individuals. Setting and design: A total 42 patients with Rheumatoid Arthritis (20 females, 22 males) in the age of 30-50 years were included, as a control, 50 matched healthy volunteers (20 females and 30 males) were involve, non of the subjects smokes nor receiving any form of drugs. Subjects with any acute infections or with coexisting system disease such as coronary artery disease, hypertension or chronic renal failure were excluded. Methods: Antioxidant enzymes in erythrocyte , superoxide dismutase (SOD), glutathione peroxidase (Gpx) and Cayalase (CAT) was measured. Malondialdehyde (MDA) along with Copper, Zink and Iron are measured by using the serum of the patients and control group. Results: The serum level of MDL was higher in RA patients compared with the control group, the differences among both groups was statistically significant (P < 0.001) Conclusion There is oxidative stress in RA patients evidenced by increased serum MDA and decreased antioxidant enzymes activity.
Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL, Jameson JL, Loscalzo J. Harrison’s Principles of Internal Medicine. 17th Edition, McGrawHill, New York, pp. 2083-2092, 2008.
Staron A, Makosa G , Koter-Michalak M. Oxidative stress in erythrocytes from patients with rheumatoid Arthritis :Rheumatol Int 2012: 32:331–334
Gutteridge IBH. Antioxidants in nutrition . Health and Disease. Oxford Univer. Press (1994)
Duchen MR. Roles of mitochondria in health and disease . Diabetes, 2004; 53 Suppl 1: S96 – 102 .
Boff J, Min DB. Chemistry and reaction of singlet oxygen in foods . Comp Rev Food Sci Saf, 2002 ; 1: 58 – 72 .
Girotti A. Lipid hydroperoxide generation , turnover , and effector action in biological system . J. Lipid Res, 1998; 39: 1529- 1539 .
Halliwell B, Gutteridge JMC. Free radicals in biology and medicine , 2nd ed. Oxford, Oxford University Press, 1989.
Georgieva N & Gadjeva V,. Beneficial effect of isonicotinoylhydrazones on isoniazid – induced disruption of ecological oxidative balance . Medical Hypotheses, 2005 ;64, No 3, 662 – 663.
.Barbosa KB, Bressan J, Zulet MA, Martínez Hernández JA [Influence of dietary intake on plasma biomarkers of oxidative stress in humans] An Sist Sanit Navar. 2008 Sep;31(3):259-80
Wierkot J, Mie˛dzybrodzki R. The meaning of the methotrekseta in monotherapy and multiple-therapy in patients with rheumatoid arthritis. Terapia nr. 2005: 3, 2(164)
Matyska-Piekarska E, Łuszczewski A, Ła˛cki J, Wawer I . The role of oxidative stress in the etiopathogenesis of rheumatoid arthritis. Postepy Hig Med Dosw . 2006; 60:617–623
Guidet .B and Shah SV. Amer.J. Physiol. 1989; 257 (26): 440- 445 .
Winterbourn CC; Hawkins ER, Brian M & carrel RW : The estimation of red cell soperoxide dismatase activity . J. Lab.Clin .Med. 1975; 85(2): 337-341 .
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70 : 158-69.
Aebi H . Catalase in vitro methods. Enzymol 1984;105:121–126
Mates JM. Sanchez – Jimenez FM. Role of reactive oxygen species in apoptosis Implications for cancer therapy . The International Journal of Biochemistry & Cell Biology, 2000; 32: 157-170.
Hristozov DV. Gadjeva T. Vlaykova S, Popova & Dimitrov G. . Evaluation of oxidative stress in patients with cancer . Archives of Physiology and Biochemistry , 2001; 109(4): 331 – 336 .
Dalle-Donne I, Giustarini D, Colombo R, Rossi R, Milzani A. Protein carbonylation in human diseases. Trends Mol Med. 2003; 9(4):169–176
Shaabani Y, Foroughi M, Rastmanesh R, Jamshidi A, Tajik N, Assadi O. Assessment of antioxidant nutrient intake and malondiadehyde plasma level in rheumatoid arthritis. Atherosclerosis J. 2009; 5: 1-5.
Ozkan Y , Yardým-Akaydýn S, Sepici A, Keskin E, Simsek B.Oxidative status in rheumatoid arthritis 2007, Clin Rh 26, Issue 1, pp 64-68
Hassan SZ, Gheita TA, Kenawy SA, Fahim AT, El-Sorougy IM, Oxidative stress in systemic lupus erythematosus and rheumatoid arthritis patients: relationship to disease manifestations and activity.A bdou MS Int J Rheum Dis. 2011 Oct;14(4):325-31.
. 22 Ansari S, Jaiswal G. Oxidative status in rheumatoid arthritis–A biomedical study. Antiseptic 2008: 105:429-431.
Sevens A, Guzel A, Aslan M, Hamuryudan V. Lipid, protein, DNA oxidation and antioxidant status in rheumatoid arthritis. Clin biochem. 2008May;41(7-8):538-43
Prakash B. Desai, S. Manjunatb H, Sumangalakadi K. Chetana, J. . Oxidative stress and e nzymatic antioxidant statusin rheumatoid arthritis: a case control study .European Review for Medical and Pharmacological Sciences 2010; 14: 959-967
Shah D, Wanchu A, Bhatnagar A. Interaction between oxidative stress and chemokines: possible pathogenic role in systemic lupus erythematosus and rheumatoid arthritis. Immunobiology. 2011 Sep;216(9):1010-7
Navarro-Compán V, Melguizo-Madrid E, Hernández-Cruz B, Santos-Rey K, Leyva-Prado C, González-Martín C, Navarro-Sarabia F, González-Rodríguez C. Interaction between oxidative stress and smoking is associated with an increased risk of rheumatoid arthritis: a case-control study. Rheumatology (Oxford). 2013 Mar;52(3):487-93. 12.
Anuradha B. Patil, Annasaheb Patil*, Sangeeta Shah, Mahantesh Patil . Antioxidant gap and lipid peroxidation in patients with rheumatoid arthritis: Relationship to disease manifestations and activity. Asian Pacific Journal of Tropical Disease (2012);S592-S595
Surapneni KM, Chandrasadagopan VS. Lipid peroxidation in patients with rheumatoid arthritis. Indian J Clin Biochem 2006; 23: 41-44.
. Vijayakumar D, Suresh K. and . Manoharan S Lipid peroxidation and antioxidant status in blood of Rheumatoid arthritis patients.. Indian Journal of Clinical Biochemistry, 2006, 21 (1) 104-108
Akyol O, Isci N, Temel I, Ozgocmen S, Uz E. The relationships between plasma and erythrocyte antioxidant enzymes and lipid peroxidation in patients with rheumatoid arthritis. Joint Bone Spine 2001; 68 : 311-7
DI Silvertro RA, Marten J, Skehan M. Effects of copper supplementation on ceruloplasmin and Cu/Zn superoxide dismutase in free living rheumatoid arthritis patients. J Am Coll Nutr .1992; 11: 177-180.
Jalali, M. Shahram F., Ariaeian N., Zeraat H.i, Sadeghi MR., Akhlagy A., Zyaii N., Fatehi F. , Chamary M. Blood antioxidant enzyme levels in patients with Rheumatoid Arthritis . tumj 2006, 64(8): 81-89
Jacobson GA, Ives SJ, Narkowicz C, Jones G. Plasma glutathione peroxidase (GSH-Px) concentration is elevated in rheumatoid arthritis: a case-control study. Clin Rheumatol. 2012;31(11):1543-
Samia ZH. and Tamer AG. Oxidative stress in systemic lupus erythematosus and rheumatoid arthritis patients: relationship to disease manifestations and activity. International Journal of Rheumatic Diseases 2011; 14, (4):, 325–331, October