Effect of Nosocomial Burn Bacteria in Experimental Burn Model


  • Rahman Laibi Chelab College of Education for Pure Science / Thi-Qar University
  • Haydar Kh. Al-Maliky College of Medicine / Thi-Qar University


In present study, mice burn model were used to study the complications of bacterial infected burn cases. Eighteen male albino mice were used as a burn model which divided into two mice groups burned by boiled water (scald method), then injected by physiological normal saline (negative control), two mice were infected by Pseudomonas aeruginosa 0.2 x 108  Colony Form Unit (CFU) without burn, on other hand two mice were infected by Staphylococcus aureus (0.2 x 108 CFU) without burn, these two groups considered as a positive control. Other mice divided into two groups, each one contains six mice burned and infected by nosocomial bacterial burn isolates (0.2 x 108 CFU), all groups were followed up for 7 days then killed and histopathological changes of some internal organs were examined, livers and lungs of  examined samples showed significant pathological changes in coparision to the       negative control, suggesting that these samples suffered from variety degree of hepatitis and pneumonia. These results  were due to the complications of bacteremia and septicemia of burned model.    


Brigham PA, McLoughlin E. Burn incidence and medical care use in the United States: estimates, trends, and data sources. J Burn Care Rehabil. 1996;17(2):95-107.


McCaig LF, Burt CW. National Hospital Ambulatory Medical Care Survey: emergency department summary. Adv Data. 2004;18(340):1-34.

Organization WH. The Global Burden of Disease: Geneva; Available from www. who. int/ health info / global_ burden_disease/GBD_report_2004 update_full. pdf.

Ashburn MA. Burn pain: The management of procedure-related pain. J Burn Care Rehabil. 1995;16(3 Pt 2):365-371.

Summer GJ, Puntillo KA, Miaskowski C, Green PG, Levine JD. Burn injury pain: the continuing challenge. J Pain. 2007;8(7):533-548.

Hawkins A, Maclennan PA, McGwin Jr G, Cross JM, Rue LW, 3rd. The impact of combined trauma and burns on patient mortality. J Trauma. 2005;58(2):284-288.

Corry NH, Klick B, Fauerbach JA. Posttraumatic stress disorder and pain impact functioning and disability after major burn injury. J Burn Care Res. 2010;31(1):13-25.

Taal LA, Faber AW. Burn injuries, pain and distress: exploring the role of stress symptomatology. Burns. 1997;23(4):288-290.

Iurk LK, Oliveira AF, Gragnani A, Ferreira LM. Evidências no tratamento de queimaduras. Rev Bras Queimaduras. 2010;9(3):95- 99.

O’Sullivan ST, O’Connor TPF. Immunosuppression following thermal injury: the pathogenesis of immunodysfunction. Br J Plast Surg 1997; 50:615–23.

Mooney DP, Gamelli RL. Sepsis following thermal injury. Compr Ther 1989; 15:22–9.

Rosenthal N, Brown S. The mouse ascending: perspectives for human-disease models. Nat Cell Biol. 2007;9(9):993-999.

- Ramos M, Gragnani A, Ferreira LM. Is there an ideal animal model to study hypertrophic scarring? J Burn Care Res. 2008;29(2):363-368

- Pruitt BA Jr, Goodwin CW Jr. Current treatment of the extensively burned patient. Surg Annu 1983; 15:331–64.

- Haydar, Kh., Shanan. Experimental study of bacterial burn infection in Rabbits. Ph.D. thesis, 2012

- Horton, JW; Mass, DL; White, J. and Minei, JP.Reducing susceptibility to bacteremia after experimental burn injury: a role for selective decontamination of the digestive tract. J. Appl. Physiol 2007; 102: 2207-2216.