Study of levels of malondialdehyde (MDA) among pateints with acute myocardial infarction

  • Wajdy J Majid Dep.of biochemistry,Thiqar college of medicine


SUMMARY  : Serum malondialdehyde (MDA), as an index of lipid peroxidation, and the serum enzymes creatinin kinase (CK) , lactate dehydrogenase ( LDH ), were evaluated in a group of 32 patients with acute myocardial infarction (AMI) , 12 with angina pectoris (AP), and 88 healthy subjects as a control group . MDA values were within the normal range in those with angina pectoris patients, while in those with acute myocardial infarction patients a significant increase in serum MDA  was observed in the days following the acute event, reaching a peak level in  6-8 days later, a significant relation was found between the integrated concentration-time MDA curve and the integrated serum enzymes activity curves reached during the nine days after the acute event. The "in vivo" relevance of the increased serum MDA in the post-infarct period is unknown at the present, but as lipid peroxides are known to harm cellular structures and to inhibit prostacyclin synthesis.


'Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (Malonyldialdehyde) in biochemical systems. Anal Biochem 1966; 16:359-64.

Porter NA, Prostaglandin endoperoxides. In: Prior WA, ed. Free radicals in biology. New York: Academic Press, 1980:261-94.

Smith JB, Ingerman CM, Silver MJ. Malondialdehyde formation as an indicator of prostaglandin production by human platelets. J Lab Clin Med 1976; 88:167-72.

Flamm ES, Demopoulos HB, Seligman ML, Poser RG,Ransohoff J Free radicals in cerebral ischemia. Stroke 1978;9:445-7.

Bragt PC, Bansberg JI, Bonta IL. Antiinflammatory effects of free radical scavengers and antioxidants. Further support for proinflammatory roles of endogenous hydrogen peroxide and lipid peroxides. Inflammation 1980;4:289-99.

Harker LA, Ritchie JL. The role of platelets in acute vascular events. Circulation 1980;62 suppl v: 13-8.

Ischaemic Heart disease registers: report of the fifth working group. Copenhagen, Denmark: WHO, 1971.

Wasowicz W, Neve J, Peretz A (1993) optimized steps in fluorimetric determination of thiobarbituric acid-reactive substances in serum importance of extraction PH and influence of sample preservation and storage Clin.Chem 39:2522-2526.

. Rosalki S.B , J.Lab. Clin.Med., 69,(1967) P.696-705.

. Szasz G., Gruber W, and Bemt E. Clin.Chem. , 22(1976) P 650-656.

Oliver I.T., Biochem. J., 61 (1955) P.116-122 .

Pesce A.Lactate dehydrogenase . Kaplan A et al. Clin Chem the C.V. Mosby Co. St Louis .Toronto . Princeton 1984, 1124- 117,438 .

Elvitch GR, Aronston SB, Feichtmeir TV, Enterline ML. Thin gel electrophoresis in agarosa. Am J Clin Pathol 1966;46:692-7.

. Elevitch GR. Lactate dehydrogenase isoenzymes. In: Fluorometric techniques in clinical chemistry. Boston: Little Brown and Co, 1973:223-33.

I5. Witheveen SAG, Hemiber HC, Itollaar LTH, Hesmens W. Quantitation of infarct size in man by means of plasma enzyme levels. Br Heart J 1975; 37:795.

Shimizu T, Kondo K, Hayaishi 0. The role of prostaglandin endoperoxides in the serum thiobarbituric acid reaction. Arch Biochem Biophys 1981; 206:271-6.

Berger HJ, Zaret BL, Speroff L, Cohen LS, Wolfson S. Cardiac prostaglandin release during myocardial ischemia induced by atrial pacing in patients with coronary artery disease. Am J Cardiol 1977; 39:481-6.

" Wennmalm A, Chanh PH, Justand M. Hypoxia causes prostaglandin release from perfused rabbit hearts. Acta Physiol Scand 1974; 91:133-5.

Kuehl FA, Humes JL, Egan RW, Ham EA, Beveridge GC, Van Arman CG. Role of prostaglandin endoperoxide PGG2 in inflammatory processes. Nature 1977; 265:170-3.

Szceklik A, Gryglewski RJ, Domaglae B, et al. Serum lipoproteins, lipid peroxides and prostacyclin biosynthesis in patientswith coronary heart disease. Prostaglandins 1981; 22:795-807.

Brown MS, Basu SK, Falcky JR, Ho YK, Goldstein JL. The scavenger cell pathway for lipoprotein degradation: specificity of the binding site that mediates the uptake of negativelycharged LDL by macrophages. J Supramol Struct 1980; 13:67-81.

Fogelman AM, Shechter I, Seager J, Holom M, Child JS, Edwards PA. Malondialdehyde alteration of low density lipoproteins leads to cholesteryl ester accumulation in human monocyte-macrophages. Proc Natl Acad Sci USA 1980; 77:2214-8.

Tappel AL. Lipid peroxidation damage to cell components. Fed Proc 1973; 32:1870-4.

Kontos HA, Wei EP, Poulishock JT, Dietrich WD, Magiera CJ, Ellis EF. Cerebral arteriolar damage by arachidonic acid and prostaglandin G2. Science 1980; 209:1242-5.

Gryglewski RJ. Prostaglandins, platelets and atherosclerosis. CRC Crit Rev Biochem 1980;7 :291-338.Requests for reprints to: Dr Justo Aznar, Departamentode Biopatologia.