Antifungal Activities of Alcoholic and Aqeuous Extracts of punica granatum against Some Non-Dermatophytic Fungi
Abstract
A clinical and mycological study of superficial mycosis was conducted on 23 cases (7 males and 16 females) , and collected from patients (5-50) years old. Direct microscopy by KOH (potassium hydroxide) mount and culture was undertaken to isolate the fungal pathogen in each case. Non-dermatophyte molds were isolated from 18 cases (78.26%) and 21 isolates were identified from these cases ; 10 isolates Candida albicans (47.61%), 5 isolates Rhizopus stolonifer (23.8%), 2 isolates Penicillium sp. and 2 isolates Aspergillus nidulans (9.5%) respectively, 1 isolate Alternaria alternata and1 isolate Fusarium sp. (4.7%) respectively . Alcoholic & aqueous extracts of the punica granatum (Pomegranate) peels were prepared. The anti-fungal activity of the extracts was evaluated on isolated fungi by means of the agar-well diffusion assay. The Minimum inhibitory concentrations were 10-300 mg/ml against isolated fungi. Their was little difference between the activities of alcoholic extract & aqueous extract. These results suggest the Pomegranate Peels extract which contains active constituents as a promising anti-fungal agent.References
- •Abalaka, ME ; Olonitola, OS;Onaolapo, JA and Inabo, HI. (2009). Determination of Activity, Time Survival and Pharmacokinetics of Extracts From Momordica charantia on Some Bacterial Pathogens. Int. Jor. P. App. Scs., 3(3):6-13
- •Aggarwal, A; Arora, U and Khanna S.( 2002).Clinical and Mycological Study of Superficial Mycoses in Amritsar. Indian J dermatol; 47(4): 218 – 220.
- •Al-Khazaragi, S.M. (1991) .Biopharmacological study of Artemisia herba -alba .MSC.Thesis .Univ .Baghdad .
- •Baker, C. N. and Thornsberg, C. H. (1983). Inoculum standardization in antimicrobial susceptibility tests:Evaluation of overnight age culture. J. Chin. Microbiol., 17:140-457.
- •Baran, R; Hay, R; Haneke, E and Tosti, A.( 2006).Onychomycosis. The current approach to Diagnosis and Therapy. 2nd. Ed. Boca Raton, FL, USA: Taylor & Francis Group;
- •Barriada-Pereira, M.;Concha-Graña, E.; González-Castro, M. J.; Muniategui-Lorenzo, S.; López-Mahía, P.; Prada-Rodríguez, D. and Fernández-Fernández, E. (2003). Microwave-assisted extraction versus Soxhlet extraction in the analysis of 21 organochlorine pesticides in plants. Elsevier B.V. J Chromatogr. 1008: 115-122.
- •Chander J. (2009).Superficial Cutaneous Mycosis. In: Textbook of Medical Mycology, 2nd edition, Mehta Publisher, New Delhi, India; 92-147.
- •Collee, JG; Fraser, AG; Marmion, BP and Simmons, A. ( 1996). Fungi. In: Mackie McCartney Practical Medical Microbiology, 14th ed. Churchill Livingstone, UK: 695-717.
- •Cravatto, G ; Boffa, L; Genzini, L and Garella D (2010). Phytotherapeutics : Anevaluation of the potential of 1000 plants. J. Clin. Pharm. Ther., 35: 11-48.
- •Egharevba, HO and Kunle, OF. (2010). Preliminary Phytochemical and Proximate Analysis of the leaves of Piliostigma thionningii (Schumach.) Milne-Redhead. Ethnobotanical Leaflets, 14: 570-77.
- •Endo, E. H.; Cortez, D. A.; Ueda-Nakamura, T.; Nakamura, C. V.and Dias Filho, B. P. (2010). Potent antifungal activity of extracts and pure compound isolated from pomegranate peels and synergism with fluconazole against Candida albicans. Res. Microbiol. 161, 534−540.
- •Gil, M. I.; Tomas-Barberan, F. A.; Hess-Pierce, B.; Holcroft, D. M.and Kader, A. A. (2000).Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric. Food Chem., 48: 4581−4589.
- •Harborne,J.B.(1984).Pytochemical methods :Aguide to modern techniques of plant analysis.2nd ed.Chapman and Hall,London .UK.288p.
- •Jayaprakasha, G. K.; Negi, P. S. and Jena, B. S. (2006). Antimicrobial activities of pomegranate. In Pomegranates: Ancient Roots to Modern Medicine; Seeram, N. P., Schulman, R. N., Heber, D., Eds.; CRC Press: Boca Raton, FL: 167−183.
- •Jawetz, M and Adelbergs (2001). Medical microbiology 22 Ed , p201 Lange Medical Books/ McGraw-Hill. Medical Publishing Division.
- •Kim, ND; Mehta, R;Yu, W; Neeman, I; Livney, T; Amichay A, et al. (2002).Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. Breast Cancer Res Treat. Feb;71(3):203–17.
- •Koneman, EW; Allen, SD; Janda, WM; Schreckenberger, Pc and Winn, WC. (1997). Mycology. In: Color Atlas and Text book of Diagnostic Microbiology, 5th ed. Lippincott Williams and Wilkins, USA: 983 – 1069.
- Lansky, E. P. and Newman, R. A. ( 2007). “Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer,” Journal of Ethnopharmacology, 109( 2): 177–206.
- Naz, S. ; Siddiqi, R. ; Ahmad, S. Rasool, S. A. and Sayeed, S. A. (2007). “Antibacterial activity directed isolation of compounds from Punica granatum,” J . Food Sci, 72( 9): 341–345.
- Oloyede,O.O.(2005).Chemical profile of unripe pulp of Carica papaya. Pak. J.Nutri. 4(6):379-381.
- •Osorioa, E.; Floresa, M.; Hernndeza, D.; Venturab, J.; Rodrgiez, R. and Aguilar, C. N.( 2010).Biological efficiency of polyphenolic extracts from pecan nuts shell (Carya Illinoensis), pomegranate husk (Punica granatum) and creosote bush leaves (Larrea tridentata Cov.) against plant pathogenic fungi. Ind. Crops Prod. 31, 153−157.
- Padhye, A.A. and Weitzman, I. (2005).The dermatophytes, Topley and Wilson’s Microbiology and Microbial infections, 10th ed, London:782-815.
- Parmar, H. S. and Kar, A. (2007).“Protective role of Citrus sinensis, Musa paradisiaca, and Punica granatum peels against diet-induced atherosclerosis and thyroid dysfunctions in rats,” Nutrition Research, 27, ( 11): 710–718.
- •Perez, C., Pauli, M. and Bazerque, P. (1990). An antibiotic assay by agar-well diffusion method. Acta Biologiae et Medecine Experimentaalis, 15:113-115.
- Related, A.; LinksHeber, D. ; Seeram, N. P. et al., ( 2007). “Safety and antioxidant
activity of a pomegranate ellagitannin-enriched polyphenol dietary supplement in overweight individuals with increased waist size,” J. Agric. & Food Chemi., 55: 10050–10054.
- Ribereau – Gayon , P. (1972) . Plant phenolics . 7 th ed . Oliver and Boyed . Endinbarg . 254 p .
- Ricci, D; Giamperi, L; Bucchini, A. and Fraternale, D. ( 2006). “Antioxidant activity of Punica granatum fruits,” Fitoterapia, 77( 4): 310–312.
- •Saad Sabbar Dahham, Mir Naiman Ali, Hajera Tabassum and Mazharuddin Khan ( 2010).Studies on Antibacterial and Antifungal Activity of Pomegranate (Punica granatum L.) American-Eurasian J. Agric. & Environ. Sci., 9 (3): 273-281.
- •Samy, R. P. and Gopalakrishnakone, P. (2008).Therapeutic potential of
plants as anti-microbials for drug discovery. Evidence-Based Compl. Alt.
Med. 7: 283−294.
- •Sánchez-Lamar, A. ; Fonseca, G Fuentes . J. L. et al., ( 2007). “Assessment of the genotoxic risk of Punica granatum L. (Punicaceae) whole fruit extracts,” Journal of Ethnopharmacology, 115( 3): 416–422.
- •Siham S. Shaokat , Hamoudi A. Hameed , Hassan J. MohammadSiham, S.; Shaokat, Hamoudi A.; Hameed and Hassan J.Mohammad(2007). Anti-fungal Activity of Punica granatum I.peels Powder and Extracts from Pathogenic Samples. Iraqi J.Pharm.Sci., 16 (2):12.
- •Tayel, A. A.; El-Baz, A. F.; Salem, M. F. and El-Hadary, M. H. (2009).
Potential applications of pomegranate peel extract for the control of
citrus green mold. J. Plant Dis. Prot. 116:252−256.
- •Trease,G.E. and Evans,W.C.(1996).A text book of pharmacognosy.14th ed. Bailliere Tindall Ltd.London.
- •Vasconcelos, LC; Sampaio, MC; Sampaio, Fc and Higino JS. (2003). Use of Punica granatum as an antifungal agent against candidosis associated with denture stomatitis. Mycoses;46(5–6):192–6.
- •Vasconcelos, LC; Sampaio, FC; Sampaio, MC; Pereira Mdo, S; Higino JS and Peixoto MH. (2006). Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans. Braz Dent J. 17(3):223–7.
- -Voravuthikunchai, S. P. ; Sririrak, T. ; Limsuwan, S. ; Supawita, T. ; Iida, T. and Honda, T. ( 2005). “Inhibitory effects of active compounds from Punica granatum pericarp on verocytotoxin production by enterohemorrhagic Escherichia coli O157:H7,” J Health Science, 51( 5): 590–596.
- -•Zhang, J. ; Zhan, B. ; Yao, X. ; Gao, Y. and Shong, J. (1995). “Antiviral activity of tannin from the pericarp of Punica granatum L. against genital Herpes virus in vitro,” Zhongguo Zhong yao za zhi, 20( 9): 556–576.
