Phenotypic Discrimination of Antibiotic Resistance Among P. Aeruginosa Collected from Burn Sample

Authors

  • Raghad A. Ridin Department of Quality Assurance &Academic Performance. Presidency /University of Thi Qar, Iraq

Abstract

Pseudomonas aeruginosa is characterized by that it is associated with many morbidities and
deaths for people with immunodeficiency, as well as people who visit hospitals frequently and
patients with burns, especially severe ones, because the bacteria are Gram-negative, opportunistic,
and cause severe infections. The study included 45 samples that isolated the advanced aeruginosa
bacteria from moderately burned men and women, their ages ranged between 12 to 60 years. The
purpose of the study was to investigate the susceptibility of P. aeruginosa to resistance to some
antibiotics for the purpose of controlling the incidence of wound infection in patients with burns.
The phenotypic pattern of the bacteria under study shows that there is severe resistance to some
antibiotics, while the bacteria are unable to resist some other antibiotics.

References

Maroui, I., Barguigua, A., Aboulkacem, A., Elhafa, H., Ouarrak, K., Sbiti, M., ... & Belhaj, A.

(2017). Clonal analysis of clinical and environmental Pseudomonas aeruginosa isolates from Meknes

region, Morocco. Polish Journal of Microbiology, 66(3).

Schaber, J. A., Hammond, A., Carty, N. L., Williams, S. C., Colmer-Hamood, J. A., Burrowes, B.

H., ... & Hamood, A. N. (2007). Diversity of biofilms produced by quorum-sensing-deficient clinical

isolates of Pseudomonas aeruginosa. Journal of medical microbiology, 56(6), 738-748.

Alikhani, M. Y., Parsavash, S., Arabestani, M. R., & Hosseini, S. M. (2017). Prevalence of antibiotic

resistance and class 1 integrons in clinical and environmental isolates of Pseudomonas

aeruginosa. Avicenna Journal of Clinical Microbiology and Infection, 4(4), 12086-12086.

Davane, M., Suryawanshi, N., Pichare, A., & Nagoba, B. S. (2014). Pseudomonas aeruginosa from

hospital environment. Journal of Microbiology and Infectious Diseases, 4(01).

de Abreu, P. M., Farias, P. G., Paiva, G. S., Almeida, A. M., & Morais, P. V. (2014). Persistence of

microbial communities including Pseudomonas aeruginosa in a hospital environment: a potential health

hazard. BMC microbiology, 14(1), 1-10.

Karami P., Mohajeri P., Mashouf R. Y., Karami M., Yaghoobi M. H., Dastan D., Alikhani M. Y.

(2019). Molecular characterization of clinical and environmental Pseudomonas aeruginosa isolated in a

burn center, Saudi Journal of Biological Sciences, Volume 26, Issue 7, Pages 1731-1736.

Hassuna, N. A., Mohamed, A. H. I., Abo-Eleuoon, S. M., & Rizk, H. A. W. A. (2015). High

prevalence of multidrug resistant Pseudomonas aeru. Archives of Clinical Microbiology, 6(4).

Karami, P., Bazmamoun, H., Sedighi, I., Nejad, A. S. M., Aslani, M. M., & Alikhani, M. Y. (2017).

Antibacterial resistance patterns of extended spectrum β-lactamase-producing enteropathogenic

Escherichia coli strains isolated from children. Arab Journal of Gastroenterology, 18(4), 206-209.

Schaber, J. A., Hammond, A., Carty, N. L., Williams, S. C., Colmer-Hamood, J. A., Burrowes, B.

H., ... & Hamood, A. N. (2007). Diversity of biofilms produced by quorum-sensing-deficient clinical

isolates of Pseudomonas aeruginosa. Journal of medical microbiology, 56(6), 738-748.

Alikhani, M. Y., Tabar, Z. K., Mihani, F., Kalantar, E., Karami, P., Sadeghi, M., ... & Farajnia,

S. (2014). Antimicrobial resistance patterns and prevalence of blaPER-1 and blaVEB-1 genes among

ESBL-producing Pseudomonas aeruginosa isolates in West of Iran. Jundishapur Journal of

Microbiology, 7(1).

Safari, M., Alikhani, M. Y., Arabestani, M. R., Kakhki, R. K., & Jafari, R. (2014). Prevalence of

Metallo-β-lactamases encoding genes among pseudomonas aeruginosa strains isolated from the bedridden

patients in the intensive care units. Avicenna Journal of Clinical Microbiology and Infection, 1(1), 19216-

Rasamiravaka, T., Labtani, Q., Duez, P., & El Jaziri, M. (2015). The formation of biofilms by

Pseudomonas aeruginosa: a review of the natural and synthetic compounds interfering with control

mechanisms. BioMed research international, 2015.

Rasamiravaka, T., Vandeputte, O. M., Pottier, L., Huet, J., Rabemanantsoa, C., Kiendrebeogo,

M., ... & El Jaziri, M. (2015). Pseudomonas aeruginosa biofilm formation and persistence, along with the

production of quorum sensing-dependent virulence factors, are disrupted by a triterpenoid coumarate ester

isolated from Dalbergia trichocarpa, a tropical legume. PloS one, 10(7), e0132791.

Chemaly, R. F., Simmons, S., Dale Jr, C., Ghantoji, S. S., Rodriguez, M., Gubb, J., ... & Stibich,

M. (2014). The role of the healthcare environment in the spread of multidrug-resistant organisms: update

on current best practices for containment. Therapeutic advances in infectious disease, 2(3-4), 79-90.

Oliveira, A. C. D., & Damasceno, Q. S. (2010). Surfaces of the hospital environment as possible

deposits of resistant bacteria: a review. Revista da Escola de Enfermagem da USP, 44(4), 1118-1123.

Sadikot RT, Blackwell TS, Christman JW, Prince AS.( 2005). Pathogen–host interactions

in Pseudomonas aeruginosa pneumonia. Am J Respir Crit Care Med.;171(11):1209–1223.

Lister PD, Wolter DJ, Hanson ND. (2009). Antibacterial-resistant Pseudomonas aeruginosa: clinical

impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol

Rev.;22(4):582–610.

Pitten, F. A., Panzig, B., Schröder, G., Tietze, K., & Kramer, A. (2001). Transmission of a

multiresistant Pseudomonas aeruginosa strain at a German University Hospital. Journal of Hospital

Infection, 47(2), 125-130.

Climo, M. W., Pastor, A., & Wong, E. S. (1997). An outbreak of Pseudomonas aeruginosa related to

contaminated urodynamic equipment. Infection Control & Hospital Epidemiology, 18(7), 509-510.

Moolenaar, R. L., Crutcher, J. M., San Joaquin, V. H., Sewell, L. V., Hutwagner, L. C., Carson,

L. A., ... & Jarvis, W. R. (2000). A prolonged outbreak of Pseudomonas aeruginosa in a neonatal intensive

care unit did staff fingernails play a role in disease transmission?. Infection Control & Hospital

Epidemiology, 21(2), 80-85.

Hassuna, N. A., Mohamed, A. H. I., Abo-Eleuoon, S. M., & Rizk, H. A. W. A. (2015). High

prevalence of multidrug resistant Pseudomonas aeru. Archives of Clinical Microbiology, 6(4).

luit, A. C., J. Verhoef, F. J. Schmitz, and The European SENTRY Participants (2001). Frequency

of isolation and antimicrobial resistance of gram-negative and gram-positive bacteria from patients in

intensive care units of 25 European university hospitals participating in the European arm of the SENTRY

Antimicrobial Surveillance Program 1997-1998. Eur. J. Clin. Microbiol. Infect. Dis.20:617-625.

Livermore, D. M.2002. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa:

our worst nightmare? Clin. Infect. Dis.34:634-640.

Stanković-Nedeljković, N., Tiodorović, B., Kocić, B., Ćirić, V., Milojković, M., & Waisi, H. (2015).

Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs. Vojnosanitetski

pregled, 72(11), 996-1003.

Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G., ... &

Monnet, D. L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an

international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology

and infection, 18(3), 268-281.

Jovcic, B., Lepsanovic, Z., Suljagic, V., Rackov, G., Begovic, J., Topisirovic, L., & Kojic, M.

(2011). Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from

Serbia. Antimicrobial agents and chemotherapy, 55(8), 3929-3931.

Sardelic, S., Bedenic, B., Colinon-Dupuich, C., Orhanovic, S., Bosnjak, Z., Plecko, V., ... &

Rossolini, G. M. (2012). Infrequent finding of metallo-β-lactamase VIM-2 in carbapenem-resistant

Pseudomonas aeruginosa strains from Croatia. Antimicrobial agents and chemotherapy, 56(5), 2746-2749.

Hassuna, N. A., Mohamed, A. H. I., Abo-Eleuoon, S. M., & Rizk, H. A. W. A. (2015). High

prevalence of multidrug resistant Pseudomonas aeru. Archives of Clinical Microbiology, 6(4).

de Almeida, K. D. C. F., Calomino, M. A., Deutsch, G., de Castilho, S. R., de Paula, G. R., Esper,

L. M. R., & Teixeira, L. A. (2017). Molecular characterization of multidrug-resistant (MDR)

Pseudomonas aeruginosa isolated in a burn center. Burns, 43(1), 137-143.

Taylor, P. K., Yeung, A. T., & Hancock, R. E. (2014). Antibiotic resistance in Pseudomonas

aeruginosa biofilms: towards the development of novel anti-biofilm therapies. Journal of biotechnology,

, 121-130.

Gurung, J., Khyriem, A. B., Banik, A., Lyngdoh, W. V., Choudhury, B., & Bhattacharyya, P.

(2013). Association of biofilm production with multidrug resistance among clinical isolates of

Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit. Indian journal of critical

care medicine: peer-reviewed, official publication of Indian Society of Critical Care Medicine, 17(4), 214.

Yekani, M., Memar, M. Y., Alizadeh, N., Safaei, N., & Ghotaslou, R. (2017). Antibiotic resistance

patterns of biofilm-forming Pseudomonas aeruginosa isolates from mechanically ventilated patients.

International Journal of Scientific Study, 5(5), 1-5.

Karami, P., Mohajeri, P., Yousefi Mashouf, R., Karami, M., Yaghoobi, M. H., Dastan, D., &

Alikhani, M. Y. (2019). Molecular characterization of clinical and environmental Pseudomonas aeruginosa

isolated in a burn center. Saudi journal of biological sciences, 26(7), 1731–1736.

Haeili, M., Ghodousi, A., Nomanpour, B., Omrani, M., & Feizabadi, M. M. (2013). Drug resistance

patterns of bacteria isolated from patients with nosocomial pneumonia at Tehran hospitals during 2009-

The Journal of Infection in Developing Countries, 7(04), 312-317.

Singh, A., Goering, R. V., Simjee, S., Foley, S. L., & Zervos, M. J. (2006). Application of molecular

techniques to the study of hospital infection. Clinical microbiology reviews, 19(3), 512-530.

Haeili, M., Ghodousi, A., Nomanpour, B., Omrani, M., & Feizabadi, M. M. (2013). Drug resistance

patterns of bacteria isolated from patients with nosocomial pneumonia at Tehran hospitals during 2009-

The Journal of Infection in Developing Countries, 7(04), 312-317.

Cunha BA. (2002). Pseudomonas aeruginosa: resistance and therapy. Semin Respir Infect.

Dambrauskiene A, Adukauskiene D, Jeroch J, Vitkauskiene A. (2009). Pseudomonas aeruginosa

padermiu, sukelusiu bakteriemija, sasajos su infekcijos zidiniu ir ju atsparumas antibiotikams

[Pseudomonas aeruginosa bacteremia: associations with a source of infection and antibiotic resistance].

Medicina (Kaunas).;45(1):1-7. Lithuanian. PMID: 19223699

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2024-01-04

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