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June 27, 2026
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Abstract

Background: Urinary tract infection UTI is one of the most common bacterial infection , predominantly caused by E.Coli this case control study aimed to evaluate demographic characteristic ,hematological parameters ,serum interleukin IL-6 and associated of TLR4  gene polymorphism (rs4986790 and rs4986791) with susceptibility to UTI Aim:To evaluated demographic characteristics inflammatory and hematological parameters ,serum ;IL-6 levels and the association of TLR4 gene polymorphism (rs 4986790 and rs 4986791). Methods:A total of 66 UTI patients and 55 healthy control were enrolled demographic data ,complete blood count , C–reactive protein CRP , urine analysis finding and serum IL-6 level were measured .TLR4 polymorphism were determining using PCR-RFLP , and Statistical analysis including odds ratios , 95% confidences intervals and effect size estimation .Results: No significant differences were found between patients and control healthy  age or sex distribution (p > 0.05). UTI  patients exhibited significantly elevate white blood cell counts, urinary red blood,  neutrophil percentages, CRP levels, cells, and pus cells compared to controls (p < 0.001). Hemoglobin levels were lower in patients; however, this difference was not statistically significant (p = 0.08). serum IL-6 were markedly increased in UTI patients (47.9 ± 17.6 pg/mL) .compared with control (10.4 ± 4.8 pg/mL), indicated strong statistical  significant  p < 0.001 and a large effect size (Cohen’s d = 2.65). Genotype and allele distributions of TLR4 rs4986790 (Asp299Gly) and rs4986791 (Thr399Ile) did not demonstrate  statistically significant associations with UTI susceptibility (p > 0.05) .Conclusion: UTI patients showed significant systemic and local inflammatory responses compared to healthy controls. TLR4 polymorphisms (rs4986790 and rs4986791) were not significantly associated with UTI susceptibility. These results highlight the diagnostic value of inflammatory markers, particularly IL-6, and suggest further studies on host genetic factors.

Keywords

IL-6 , ELISA E. coli TLR-4 SNP Polymorphism

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