Molecular Identification for Entamoeba Species by Gene Sequence in Thi_Qar Province / lraq
Abstract
Background: Microscopically, E.moshkovskii and E. dispar cannot be distinguished fromthe pathogenic species E. histolytica. A common molecular biology approach for
amplifying many targets simultaneouslyis polymerase chain reaction (PCR).
Methods: The use of various DNA extraction techniques in PCR assays for the
identification and differentiation of the three-microscopy indistinguishable Entamoeba
species in humans was investigated. The small-subunit rRNA gene's midsection was used to
create a conserved forward primer, and reverse primers were created using signature
sequences unique to each of thes three Entamoebaspecies. Results : The current Study
used PCR for the 296 microscopically positive patients .the molecular study showed that of
144 ( 48.64%) were E. histolytica, 91(30.74%) were E.dispar and 52(17.56) were
E.moskovski.and association of E.histolytica an E.dispar was recorded for 3.04% .and
gene Sequence.
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