Detection of Trichomonas vaginalis among women with abnormal vaginal discharge by PCR technique targeting TVK3 and TVK7 genes in Basrah province

  • Amal KH. Khalaf

Abstract

      A total of 552 samples of vaginal discharge were collected from women that visited the Maternity and Pediatrics hospital in Basrah Province .Microscopic examination of vaginal discharge revealed that 4.1 % of these women were infected with Trichomoniasis .      In present diagnostic study, two technique were used to diagnosed the same sample for the first time in Iraq , these include microscopic examination ( wet preparation ) and  Polymerase chain reaction (PCR) .PCR technique was the highest sensitivity ( 100 % ) which diagnosed three samples were considered negative in the other diagnostic technique and microscopic recorded sensitivity 88.4 %  .In the same time diagnosis by vaginal discharge were found to be highly percent ( 100 % ) than the urine (1.9%) .    

References

Abdus, M. and Talukder,S.(1986) . Trichomonas vaginalis in Riyadh. Saudi Arabia . Saudi. Med . J. , 7 : 96.

Al-Ani ,S.F.I.H.; AI-Hadithi, I.A.W. and Al-Hadithi, R.J.K. (2001). Factors affecting vaginal trichomoniasis among women in Ramadi. J. Basrah. Res., 27 : 24- 35.

Al – Saadi, K.M. ( 2003 ) . Microbiological study of bacterial vaginosis and its correlate with the risk of urinary tract infection among pregnant women . M.Sc. Thesis , Med. Coll. Kufa. Univ.

Bowden, F.J. and Garnett, G.P. ( 2000) . Trichomonas vaginalis epidemiology : parametrerising and analyzing a model of treatment intervention . Sex. Transm. Infect., 76 : 248 – 256 .

Caterall, R.D. ( 1970 ) . Diagnosis of vaginal discharge . Br. J. Vener . Dis., 46 : 122 – 124 .

Davis , A.G. and Clay, J.C. (1992) . Prevalence of sexually transmitted infection in women alleging rape . sex. Transm. Dis., 10 : 298 – 300

Dettovitz, J.A. ; Kelly, P ; Feldman, J. ; Sierra, M.F. ; Clark, L. and Bromberg, J. ( 1994 ) . Sexually transmitted disease , sexual behavior , and cocaine use in inner – city women . Am. J. Epidemiol., 140 : 1125 – 1134 .

Fiori, P.L. ; Rappelli, P. ; Rocchigiani, A.M. and Cappuccinelli, P. (1993). Trichomonas vaginalis haemolysis : evidence of functional pores formation on red cell membranes . FEMS Microbiol. Lett. 109 : 13 – 18 .

Graves, A. and Gardner, W.A. ( 1993 ) . Pathogenicity of Trichomonas vaginalis . Clin. Obestet. Gynecol. , 36 : 145 – 152 .

Gani , Z.H. (2000). Urogenital trichomoniasis among women in Basrah . M. Sc. Thesis , coll. Med. Univ. Basrah , 91 pp.

Karyakarte , R. and Damle , A. (2003). Medical parasitology . Calcuta art studio , India : 288 pp

Kengne , P. ; Veas , F. ; Vidal , N. ; Rey , J. and Cuny , G. (1994). Trichomonas vaginalis : repeated DNA target for highly sensitive and specific polymerase chain reaction diagnosis . Cell. Mol. Biol. , 40 : 819 – 823.

Lo , M. ; Reid, M. and Brokenshire , M. ( 2002) . Epidemiological features of women with trichomoniasis in Auckland sexual health clinics : 1998 – 1999 . NZ . Med. J., 115 : 119 – 126.

Madani , T.A. ( 2006) . Sexually transmitted infection in Saudi Arabia . BMC .Infect. Dis ., 6 : 218 – 225 .

Petrin , D. ; Delgaty , K. ; Bhatt , R. and Garber , G. (1998). Clinical and microbiological aspect of Trichomonas vaginalis . Clin. Microbiol. Rev. , 11: 300 – 317.

Riley , D.E. ; Roberts , M.C. ; Takayama , T. and Krieger , J.N. (1992). Development of a polymerase chain reaction – based diagnosis of Trichomonas vaginalis . J. Clin. Microbiol. , 30: 465 – 472.

Rosenberg , M.J. ; Davidson, A.J. ; Chen, J.H. ; Judson, F.N. and Douglas, J.M. ( 1999 ) . Barrier contraceptive and sexually transmitted disease in women : a comparison of female – dependent methods and condoms . Am. J. Pupli. Heal. , 82: 669 – 674.

Sambrook , J. ; Fritsh , E.F. and Maniatis , T. (1989). Molecular cloning , alaboratory manual . 2nd ed. Cold Spring Harbor laboratory press.USA.

Swygard, H. ; Sena, A.C. ; Hobbs , M.M. and Cohen , M.S. ( 2004) . Trichomoniasis : clinical manifestation , diagnosis and management . Sex. Transm. Infect. , 80 : 91 – 95.

Schwebke , J.R. and Burgess , D. (2004). Trichomoniasis . Clin. Microbiol. Rev. , 17: 794 – 803.

Sena , A.C. ; Miller , W.C. ; Hobbs , M.M. ; Schwebke , J.R. ; Leone , P.A. ; Swygard , H. ; Atashili , J. and Cohen , M.S.(2007). Trichomonas vaginalis infection in male sexual partners : Implication for diagnosis , treatment and prevention . Clin. Infect. Dis. , 44 : 13 – 22.

Verteramo, R. ; Calzolari, E. ; Degener, A.M. ; Masciangelo, R. and Patella , A. ( 2008 ) . Trichomonas vaginalis infection : Risk indicators among women attending for routine gynecologic examination . J. Obstet. Gynecol. Res. , 34: 233 – 237.

Yasmeench , M.N. ( 1998 ) . Reproductive morbidity in the country side of Domascus . Domascus Univ. J. Med.Sci. 14 : 119 – 135 .

Yazar , S. ; Dagci , H. ; Aksoy , U. ; Ustun , S. ; Akisu , C. ; Mucide , AK. and Daldal , N. (2002). Frequency of Trichomonas vaginalis among women having vaginal discharge in Izmir , Turkey . Inonu. Univ. Tip. Fakut. Derg. , 9: 159 – 161.

Published
2021-03-22
Section
Articles